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Titolo:
Somatic embryogenesis in Asparagus officinalis can be an in vitro selection process leading to habituated and 2,4-D dependent embryogenic lines
Autore:
Limanton-Grevet, A; Jullien, M;
Indirizzi:
Lab Biol Semences, UMR 204, INRA, INAPG, F-78026 Versailles, France Lab Biol Semences Versailles France F-78026 , F-78026 Versailles, France J Marionnet GFA, Lab In Vitro, F-41230 Soings En Sologne, France J Marionnet GFA Soings En Sologne France F-41230 ings En Sologne, France Asparagus BV, NL-5960 AE Horst, Netherlands Asparagus BV Horst Netherlands NL-5960 AE NL-5960 AE Horst, Netherlands
Titolo Testata:
PLANT PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 7-8, volume: 38, anno: 2000,
pagine: 567 - 576
SICI:
0981-9428(200007/08)38:7-8<567:SEIAOC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT-REGENERATION; HERITABLE VARIATION; CULTURE; CELLS; PROTOPLASTS; GENOTYPE; MAIZE;
Keywords:
Asparagus officinalis; embryogenic capacity; habituation; mutation; somatic embryogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Jullien, M Lab Biol Semences, UMR 204, INRA, INAPG, Route St Cyr, F-78026 Versailles,France Lab Biol Semences Route St Cyr Versailles France F-78026rance
Citazione:
A. Limanton-Grevet e M. Jullien, "Somatic embryogenesis in Asparagus officinalis can be an in vitro selection process leading to habituated and 2,4-D dependent embryogenic lines", PL PHYS BIO, 38(7-8), 2000, pp. 567-576

Abstract

Long-term embryogenic lines were repeatedly obtained from nine asparagus (Asparagus officinalis L.) genotypes by the selection of ran events, which consisted of the emergence of either a few somatic embryos or an embryogeniccallus from a restricted area of a primary callus. In the first case, somatic embryos emerged from 1% of calli induced with naphtaleneacetic acid andtransferred to a medium without auxin. Isolated and subcultured on hormonefree medium, these embryos developed habituated embryogenic lines (H lines) growing by adventive embryogenesis. In the second case, 3% of primary calli developed then subcultured on 2,4-dichlorophenoxyacetic acid (2,4-D) produced a new type of friable and yellowish-white callus, constituted of clusters of globular somatic embryos which can be continuously maintained on 2,4-D (2,4-D lines). Among 2,4-D lines, two types were identified by subculturing them on hormone-free medium. Half of the 2,4-D lines were habituated and half were 2,3-D dependent. Most plants regenerated from H Lines exhibited a strong increase in embryogenic capacity compared to control plants, unlike plants regenerated from the 2,4-D dependent lines. This increased embryogenic capacity was transmitted to the progeny as a monogenic dominant trait. H lines would therefore be issued from mutation(s) occurring in vitro, conferring both the embryogenic and habituated phenotypes. On the contrary, in the 2,4-D dependent lines, the embryogenic processes appeared to remain under exogenous auxin control and no evidence of a mutational origin could be inferred from the behaviour of regenerated plants. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 16:21:48