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Titolo:
L-Deoxy-D-xylulose 5-phosphate synthase from periwinkle: cDNA identification and induced gene expression in terpenoid indole alkaloid-producing cells
Autore:
Chahed, K; Oudin, A; Guivarch, N; Hamdi, S; Chenieux, JC; Rideau, M; Clastre, M;
Indirizzi:
Fac Pharm, Lab Biol Mol & Biochim Vegetale, EA2106, F-37200 Tours, France Fac Pharm Tours France F-37200 m Vegetale, EA2106, F-37200 Tours, France
Titolo Testata:
PLANT PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 7-8, volume: 38, anno: 2000,
pagine: 559 - 566
SICI:
0981-9428(200007/08)38:7-8<559:L5SFPC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEVALONATE-INDEPENDENT PATHWAY; CATHARANTHUS-ROSEUS; ISOPRENOID BIOSYNTHESIS; ISOPENTENYL DIPHOSPHATE; HIGHER-PLANTS; 1-DEOXY-D-XYLULOSE; ACCUMULATION; CULTURES; REDUCTOISOMERASE; TRANSKETOLASES;
Keywords:
Catharanthus roseus; 1-deoxy-D-xylulose 5-phosphate synthase isopentenyl diphosphate; 2C-methyl-D-erythritol 4-phosphate pathway terpenoid indole alkaloid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Rideau, M Fac Pharm, Lab Biol Mol & Biochim Vegetale, EA2106, 31 Ave Monge, F-37200 Tours, France Fac Pharm 31 Ave Monge Tours France F-37200 37200 Tours, France
Citazione:
K. Chahed et al., "L-Deoxy-D-xylulose 5-phosphate synthase from periwinkle: cDNA identification and induced gene expression in terpenoid indole alkaloid-producing cells", PL PHYS BIO, 38(7-8), 2000, pp. 559-566

Abstract

Terpenoid indole alkaloids (TIAs) arise from the indole and the monoterpene pathways. The latter route derives from isopentenyl diphosphate (IPP). Wereport on the isolation and characterization of a cDNA (crdxs) encoding for 1-deoxy-D-xylulose 5-phosphate synthase (DXPS) in Catharnthus roseus suspension cultures. The enzyme catalyses the formation of the precursor of thenon-mevalonate pathway leading to IPP biosynthesis. Expression in Escherichia coli of the truncated DXPS lacking the putative plastid transit peptiderevealed that this pseudomature protein was active, crdxs mRNA were detected only in TIA producing-cells and the accumulation of transcripts was found to be associated with TIA production. This result corroborates recent studies obtained with a labelled precursor, which have given evidence that thenon-mevalonate pathway is involved in the biosynthesis of the precursor ofTIAs secologanin. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 12:19:37