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Titolo:
Microsatellite marker panels for use in high-throughput genotyping of mouse crosses
Autore:
Iakoubova, OA; Olsson, CL; Dains, KM; Choi, J; Kalcheva, I; Bentley, LG; Cunanan, M; Hillman, D; Louie, J; Machrus, M; West, DB;
Indirizzi:
Pfizer Global Res & Dev Alameda Labs, Alameda, CA 94502 USA Pfizer Global Res & Dev Alameda Labs Alameda CA USA 94502 a, CA 94502 USA Deltagen Inc, Menlo Park, CA 94025 USA Deltagen Inc Menlo Park CA USA 94025 ltagen Inc, Menlo Park, CA 94025 USA
Titolo Testata:
PHYSIOLOGICAL GENOMICS
fascicolo: 3, volume: 3, anno: 2000,
pagine: 145 - 148
SICI:
1094-8341(20000908)3:3<145:MMPFUI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC-MAP; COMPLEX TRAITS; LOCI; SENSITIVITY; DISSECTION; GENOME;
Keywords:
C57BL/6J; CAST/Ei; DBA/2J; quantitative trait loci analyses; congenic mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Iakoubova, OA Parke Davis Lab Mol Genet, 1501 Harbor Bay Pkwy, Alameda, CA94502 USA Parke Davis Lab Mol Genet 1501 Harbor Bay Pkwy Alameda CA USA 94502
Citazione:
O.A. Iakoubova et al., "Microsatellite marker panels for use in high-throughput genotyping of mouse crosses", PHYSIOL GEN, 3(3), 2000, pp. 145-148

Abstract

Several microsatellite genotyping panel sets have been developed that are polymorphic between C57BL/6J and CAST/Ei mice, or C57BL/6J and DBA/2J. One set of markers for each strain pair has an intermarker distance of similar to 20 cM, and a second set has an intermarker distance of 5 cM. The 20-cM set contains 105 markers for C57BL/6J X DBA/2J and 108 for C57BL/6J X CAST/Ei, divided into 13 panels. Each 5-cM set includes 350 markers arranged into45 panels. A panel contains a number of primer pairs whose fluorescently labeled PCR products can be pooled together and separated on one lane of a polyacrylamide gel. The sets are arranged by the size of the PCR product andby the type of fluorescent dye; 5-cM sets are also arranged by chromosomalregion. The 20-cM sets are most useful for full-genome scans, the 5-cM sets are useful for full-genome and/or for region-specific chromosome screens. Both sets were proven as useful tools for speed congenic development, quantitative trait loci (QTL) analysis and physical mapping. These panel sets provide a throughput of 1,536- 2,304 mouse genotypes daily per one gel-basedsystem. Whole genome scans of one animal require 13 or 48 gel lanes, with 20 cM or 5 cM density, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:20:07