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Titolo:
Bovine oocyte and embryo development following meiotic inhibition with butyrolactone I
Autore:
Lonergan, P; Dinnyes, A; Fair, T; Yang, XZ; Boland, M;
Indirizzi:
Univ Coll Dublin, Dept Anim Sci & Prod, Lyons Res Farm, Newcastle, Dublin,Ireland Univ Coll Dublin Newcastle Dublin Ireland arm, Newcastle, Dublin,Ireland Univ Connecticut, Dept Anim Sci, Storrs, CT 06269 USA Univ Connecticut Storrs CT USA 06269 Dept Anim Sci, Storrs, CT 06269 USA
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 2, volume: 57, anno: 2000,
pagine: 204 - 209
SICI:
1040-452X(200010)57:2<204:BOAEDF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERMINAL VESICLE BREAKDOWN; FOLLICULAR CELLS; GRANULOSA-CELLS; MATURATION; VITRO; RESUMPTION; 6-DIMETHYLAMINOPURINE; CYCLOHEXIMIDE; MEIOSIS; INVITRO;
Keywords:
cattle; competence IVF; meiosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Lonergan, P Univ Coll Dublin, Dept Anim Sci & Prod, Lyons Res Farm, Newcastle, Dublin,Ireland Univ Coll Dublin Newcastle Dublin Ireland le, Dublin,Ireland
Citazione:
P. Lonergan et al., "Bovine oocyte and embryo development following meiotic inhibition with butyrolactone I", MOL REPROD, 57(2), 2000, pp. 204-209

Abstract

In this study we have shown that butyrolactone I (BL-I), a potent inhibitor of cyclin-dependent kinases, inhibits meiotic resumption in bovine oocytes by blocking germinal vesicle breakdown in a dose-dependent manner. A concentration 100 mu M blocked over 60% of oocytes, while 150 mu M inhibited almost all oocytes compared to the control in which over 80% resumed meiosis. Following a second 24 hr culture under conditions permissive to normal maturation, almost all (95%) of blocked oocytes resumed meiosis and progressedto metaphase II. In terms of developmental competence, oocytes maintained in meiotic arrest for 24 hr with 100 mu M exhibited a similar capacity to develop to the blastocyst stage as nonblocked control oocytes following maturation, fertilization, and culture in vitro. Cryopreservation was employed as a tool to detect differences in the oocyte viability between blocked andcontrol oocytes. Cleavage of oocytes was significantly reduced following vitrification and activation both in BL-I treated (40.2% vs. 71.9%, P < 0.05) and the control groups (45.6% vs. 81.7%, P < 0.05). However, BL-I treatedoocytes were less likely to develop into blastocysts following vitrification (20.0% from vitrified vs 42.5% from nonvitrified cleaved oocytes, P < 0.05, based on cleaved oocytes) compared to nontreated oocytes (34.0% from vitrified vs. 42.9% from nonvitrified oocytes, P < 0.05). These results demonstrate the feasibility of maintaining bovine oocytes in artificial meiotic arrest without compromising their subsequent developmental competence and may represent a tool for improving the development of less competent oocytes. Mol. Reprod. Dev. 57:204-209, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:29:07