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Titolo:
Relationship between time of first cleavage and the expression of IGF-I growth factor, its receptor, and two housekeeping genes in bovine two-cell embryos and blastocysts produced in vitro
Autore:
Lonergan, P; Gutierrez-Adan, A; Pintado, B; Fair, T; Ward, F; De La Fuente, J; Boland, M;
Indirizzi:
Univ Coll Dublin, Dept Anim Sci & Prod, Lyons Res Farm, Newcastle, Dublin,Ireland Univ Coll Dublin Newcastle Dublin Ireland arm, Newcastle, Dublin,Ireland INIA, Dept Reprod Anim & Conservat Recursos Zoogenet, Madrid, Spain INIA Madrid Spain rod Anim & Conservat Recursos Zoogenet, Madrid, Spain
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 2, volume: 57, anno: 2000,
pagine: 146 - 152
SICI:
1040-452X(200010)57:2<146:RBTOFC>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREIMPLANTATION EMBRYOS; MOUSE EMBRYOS; MESSENGER-RNA; FACTOR LIGAND; INSULIN; MATURATION; OVIDUCT; SEX; TRANSCRIPTION; BUFFALO;
Keywords:
IGF-I; HPRT; G6PD; cattle; embryo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Lonergan, P Univ Coll Dublin, Dept Anim Sci & Prod, Lyons Res Farm, Newcastle, Dublin,Ireland Univ Coll Dublin Newcastle Dublin Ireland le, Dublin,Ireland
Citazione:
P. Lonergan et al., "Relationship between time of first cleavage and the expression of IGF-I growth factor, its receptor, and two housekeeping genes in bovine two-cell embryos and blastocysts produced in vitro", MOL REPROD, 57(2), 2000, pp. 146-152

Abstract

We have previously demonstrated that there is a clear relationship betweenthe time interval between insemination and first cleavage in vitro and thedevelopment to the blastocyst stage of bovine embryos. In addition we haveshown that this developmental ability can be linked to the stability of the mRNA for several gene transcripts measured in 2-cell bovine embryos cleaving at different times. The aim of this study was to examine the relationship between bovine embryo developmental competence. assessed in terms of time of first cleavage, and the expression of insulin-like growth factor-I (IGF-I) ligand and receptor, hypoxanthine phosphoribosyl transferase (HPRT) and glucose-6-phosphate dehydrogenase (G6PD). The expression of beta-actin was used as a reference value. No differences were observed in the mRNA expression of G6PD and HPRT genes between male and female 2-cell embryos. However, the expression of these two genes was significantly higher in female blastocysts than in male blastocysts. Moreover, when the relative amount of G6PD and HPRT mRNA detected in these groups of male and female embryos was compared, there was a significant relationship between the time of first cleavage and the relative amount of mRNA: 2-cell embryos and blastocysts derived from oocytes that cleaved at 27 and 30 hr post insemination had higher levels of mRNA for G6PD and HPRT than those that cleaved after 33 hr. IGF-I ligand and receptor was detected in all blastocysts analyzed, irrespective of stage of development or time of first cleavage. In addition, the receptorwas detected in all 2-cell embryos examined. In contrast, while IGF-I ligand was found in all 2-cell embryos that cleaved at 27 and 30 hpi, it was only found in some of those cleaving between 33 and 36 hpi and in none of those cleaving after 36 hr. in conclusion, we have demonstrated differences ingene expression in the early embryo that are reflective of differences in developmental competence between early- and late-cleaving zygotes. Mol. Reprod. Dev. 57:146-252, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 08:20:11