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Titolo:
Fission yeast living mitosis visualized by GFP-tagged gene products
Autore:
Tatebe, H; Goshima, G; Takeda, K; Nakagawa, T; Kinoshita, K; Yanagida, M;
Indirizzi:
Kyoto Univ, Grad Sch Biostudies, Dept Gene Mechanism, CREST Res Project,Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 es Project,Sakyo Ku, Kyoto 6068502, Japan
Titolo Testata:
MICRON
fascicolo: 1, volume: 32, anno: 2001,
pagine: 67 - 74
SICI:
0968-4328(200101)32:1<67:FYLMVB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINDLE POLE BODY; SISTER-CHROMATID SEPARATION; GREEN FLUORESCENT PROTEIN; SCHIZOSACCHAROMYCES-POMBE; CUT2 PROTEOLYSIS; BUDDING YEAST; ANAPHASE; NUCLEAR; CONDENSATION; LOCALIZATION;
Keywords:
green fluorescent protein; anaphase; fission yeast; S. pombe; mitosis; kinetochore; spindle pole body; tubulin; cyclin; centromere;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Yanagida, M Kyoto Univ, Grad Sch Biostudies, Dept Gene Mechanism, CREST Res Project,Sakyo Ku, Kitashirakawa Oiwakecho, Kyoto 6068502, Japan Kyoto Univ Kitashirakawa Oiwakecho Kyoto Japan 6068502 Japan
Citazione:
H. Tatebe et al., "Fission yeast living mitosis visualized by GFP-tagged gene products", MICRON, 32(1), 2001, pp. 67-74

Abstract

The fission yeast Schizosaccharomyces pombe has been used as a model organism to study cell cycle control and dynamic chromosome behavior during anaphase segregation as genetic and cytological approaches are easily amenable. To understand the role of gene products involved in these cellular events,it is important to determine intracellular localization of each gene product during the cell cycle. In this article, visualization in living cells ofseveral gene products involved in cell cycle control and sister chromatid separation is described. The genes tagged with jellyfish green fluorescent protein (GFP) include sad1(+) (encoding a spindle pole body (SPB) protein),atb2(+) (alpha-tubulin), mis6(+) (a kinetochore protein), eat1(+) (a novelactin-like protein localized in the nucleus) and cdc13(+) (a mitotic cyclin). In addition, LacI which is bound to a DNA segment containing LacO repeat sequences integrated near the centromere (cen1) is visualized. These are useful to monitor cell cycle events in living cells. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 07:11:13