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Titolo:
Cryosubstitution of frozen biological specimens in electron microscopy: use and application as an alternative to chemical fixation
Autore:
Bohrmann, B; Kellenberger, E;
Indirizzi:
F Hoffmann La Roche & Co Ltd, Preclin Res, CH-4070 Basel, Switzerland F Hoffmann La Roche & Co Ltd Basel Switzerland CH-4070 asel, Switzerland Univ Lausanne, Dept Genet & Biol Microorganisms, CH-1015 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1015 CH-1015 Lausanne, Switzerland
Titolo Testata:
MICRON
fascicolo: 1, volume: 32, anno: 2001,
pagine: 11 - 19
SICI:
0968-4328(200101)32:1<11:COFBSI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
FREEZE-SUBSTITUTION; FINE-STRUCTURE; CRYOFIXATION; IMMUNOCYTOCHEMISTRY; ULTRASTRUCTURE; DENATURATION; SECTIONS; SHAPE;
Keywords:
aggregation; cryofixation; cryosubstitution; chromatin; DNA; electron microscopy; organic solvent; physical cross-linking; resin thin sections;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Bohrmann, B F Hoffmann La Roche & Co Ltd, Preclin Res, CH-4070 Basel, Switzerland F Hoffmann La Roche & Co Ltd Basel Switzerland CH-4070 rland
Citazione:
B. Bohrmann e E. Kellenberger, "Cryosubstitution of frozen biological specimens in electron microscopy: use and application as an alternative to chemical fixation", MICRON, 32(1), 2001, pp. 11-19

Abstract

Frozen bacterial cells were low-temperature embedded after cryosubstitution at 185 K in organic solvent. Temperature elevation of cryosubstituted E. coli cells, when still in organic solvent, had no effect on the preservation of chromatin structure. The achieved stabilization was found to be established independent of the presence of the chemical fixative. Cryosubstitution without the use of a chemical fixative allows for excellent preservation of cellular ultrastructure. Beyond that, the approach is preferential for sensitive antigens in immunoelectron microscopy. We conclude that low-temperature dehydration by cryosubstitution in organic solvents is able to form stable cross-links between macromolecules by hydrophobic interactions. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:57:06