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Titolo:
Determination of malondialdehyde in biological samples by solid-phase extraction and high-performance liquid chromatography
Autore:
Bakalova, R; Mileva, M; Kotsev, C; Bardarov, V; Ribarov, S;
Indirizzi:
Med Univ Sofia, Dept Med Phys & Biophys, HPLC Lab, Sofia, Bulgaria Med Univ Sofia Sofia Bulgaria Phys & Biophys, HPLC Lab, Sofia, Bulgaria
Titolo Testata:
METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY
fascicolo: 5, volume: 22, anno: 2000,
pagine: 267 - 269
SICI:
0379-0355(200006)22:5<267:DOMIBS>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MALONALDEHYDE;
Keywords:
malondialdehyde; high-performance liquid chromatography; solid-phase extraction; precolumn derivatization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Bakalova, R Res Inst Brain & Blood Vessels, Dept Radiol & Nucl Med, 6-10 Senshu KubotaMachi, Akita 010, Japan Res Inst Brain & Blood Vessels 6-10 Senshu Kubota Machi Akita Japan 010
Citazione:
R. Bakalova et al., "Determination of malondialdehyde in biological samples by solid-phase extraction and high-performance liquid chromatography", METH FIND E, 22(5), 2000, pp. 267-269

Abstract

An analytical procedure for determination of malondialdehyde in tissue homogenates and blood serum was developed. A reaction with 2,4-dinitrophenylhydrazine is used followed by cleaning up of the derivative by solid-phase extraction. The samples were analyed by isocratic high-performance liquid chromatography (HPLC) using a narrow-bore HPLC-column. A good separation of the 1-pyrazole peak from that of 2,4-dinitrophenyl-hydrazine was observed. A high linear dependence was established by the concentration of 1-pyrazole in the range of 10-5000 ng/ml. The detection limit of the method applied fortissue homogenates and blood serum was approximately 10 ng/ml or lower, and RSD of the method was 9% (n = 8). The peak of 1-pyrazole for these samples was well separated from the other matrix peaks. Experiments carried out elevated that the solid-phase extraction might be an effective step of he sample preparation, significantly increasing the selectivity of the analysis and the life-time of the column. The method seems to be applicable for determination of malondialdehyde in different biological samples. (C) 2000 Prous Science. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:45:29