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Titolo:
CCKB/gastrin receptors mediate changes in sodium and potassium absorption in the isolated perfused rat kidney
Autore:
von Schrenck, T; Ahrens, M; de Weerth, A; Bobrowski, C; Wolf, G; Jonas, L; Jocks, T; Schulz, M; Blaker, M; Neumaier, M; Stahl, RAK;
Indirizzi:
Univ Rostock, Inst Pathol, Rostock, Germany Univ Rostock Rostock Germany niv Rostock, Inst Pathol, Rostock, Germany Univ Hamburg, Hosp Eppendorf, Dept Med, Div Nephrol, Hamburg, Germany UnivHamburg Hamburg Germany f, Dept Med, Div Nephrol, Hamburg, Germany Univ Hamburg, Hosp Eppendorf, Dept Med, Dept Clin Chem, Hamburg, Germany Univ Hamburg Hamburg Germany Dept Med, Dept Clin Chem, Hamburg, Germany
Titolo Testata:
KIDNEY INTERNATIONAL
fascicolo: 3, volume: 58, anno: 2000,
pagine: 995 - 1003
SICI:
0085-2538(200009)58:3<995:CRMCIS>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC ACINAR-CELLS; BRAIN-GUT PEPTIDES; CHOLECYSTOKININ RECEPTOR; FUNCTIONAL EXPRESSION; GUINEA-PIG; GASTRIN; CCK; CLONING; GROWTH; PENTAGASTRIN;
Keywords:
gastrin; cholecystokinin; vascular resistance; renal K and Na; gastrointestinal hormone receptors; mesangial cell growth; electrolyte transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: von Schrenck, T Univ Hamburg, Krankenhaus Eppendorf, Med Kernklin & Poliklin, Martinistr 52, D-20246 Hamburg, Germany Univ Hamburg Martinistr 52 Hamburg Germany D-20246 ermany
Citazione:
T. von Schrenck et al., "CCKB/gastrin receptors mediate changes in sodium and potassium absorption in the isolated perfused rat kidney", KIDNEY INT, 58(3), 2000, pp. 995-1003

Abstract

Background To evaluate the function of cholecystokinin B (CCKB)/gastrin receptors in the rat kidney, we identified the receptors by Northern blot andlocalized the receptors by immunohistochemistry. The functional effects ofgastrin were studied under standardized in vitro conditions using the isolated perfused kidney. Methods. Rat kidneys were mounted in an organ bath by attaching the renal artery to a perfusion system. A catheter was inserted into the renal vein and the ureter to collect samples that were analyzed for the concentrations of electrolytes. After a preperfusion period, gastrin-17-I was given via the renal artery (10(-8) to 10(-6) mol/L). Subsequently, hemodynamic parameters (for example, perfusate flow) and changes in sodium and potassium absorption were determined. All data were subjected to a nonparametric analysis of variance and, in case of significant results, to subsequent paired comparisons by the a posteriori Wilcoxon test. Results. Northern blot analysis detected CCKB receptor transcripts in total RNA isolated from kidneys. Immunohistochemistry localized CCKB receptors on tubules and collecting duct cells. Compared with controls, gastrin (10(-6) mol/L) caused a decrease in the fractional sodium reabsorption (basal 80%, 10 minutes after application of gastrin 71%, after 20 minutes 62%, P < 0.05). This effect was inhibited by the CCKB receptor antagonist L-365,260. Gastrin decreased urinary potassium excretion at 10(-8) and 10(-6) mol/L [maximal decrease at 10(-6) mol/L from baseline values (100%) to 49% after 10minutes and to 69% after 20 minutes, P < 0.05, N = 6]. This effect was also abolished by the CCKB receptor antagonist L-365,260. Gastrin (10(-6) mol/L) reduced perfusate flow by 31% (P < 0.05). Conclusions. CCKB receptors are expressed in the rat kidney on tubules andcollecting ducts. These receptors mediate changes in renal potassium and sodium absorption. Tn addition, gastrin causes a decrease in perfusate how, indicating that CCKB receptors might also modulate vascular resistance in the kidney.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 16:27:20