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Titolo:
Thermodynamic analysis of Bacillus cereus oligo-1,6-glucosidase and its cumulatively proline-introduced mutant proteins by differential scanning calorimetry
Autore:
Watanabe, K; Fujita, Y; Usami, M; Takimoto, A; Suzuki, Y;
Indirizzi:
Kyoto Prefectural Univ, Dept Appl Biochem, Lab Microbial Physiol & Appl Microbiol, Sakyo Ku, Kyoto 606, Japan Kyoto Prefectural Univ Kyoto Japan 606 obiol, Sakyo Ku, Kyoto 606, Japan
Titolo Testata:
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
fascicolo: 1-3, volume: 10, anno: 2000,
pagine: 257 - 262
SICI:
1381-1177(20000904)10:1-3<257:TAOBCO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN LYSOZYME; CONFORMATIONAL STABILITY; THERMOSTABILITY; RESIDUES; RULE; SUBSTITUTIONS; DENATURATION; SITES;
Keywords:
Bacillus cereus oligo-1,6-glucosidase; proline-introduced; differential scanning calorimetry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, Y Kyoto Prefectural Univ, Dept Appl Biochem, Lab Microbial Physiol& Appl Microbiol, Sakyo Ku, Kyoto 606, Japan Kyoto Prefectural Univ Kyoto Japan 606 yo Ku, Kyoto 606, Japan
Citazione:
K. Watanabe et al., "Thermodynamic analysis of Bacillus cereus oligo-1,6-glucosidase and its cumulatively proline-introduced mutant proteins by differential scanning calorimetry", J MOL CAT B, 10(1-3), 2000, pp. 257-262

Abstract

Differential scanning calorimetry (DSC) was used to characterize thermodynamically the stability of Bacillus cereus ATCC7064 oligo-1,6-glucosidase (dextrin 6-alpha-D-glucanohydrolase, EC 3.2.1.10) and its proline-introduced mutants. DSC analysis revealed that the free energy change of unfolding forthe mutants cumulatively increased as the number of introduced proline residues increased. The resulting increase in the free energy change was ascribed to the concomitant decrease in the entropy change of polypeptide backbone unfolding in the mutants. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 23:02:16