Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Prevalence and genetic diversity of human caliciviruses (HuCVs) in Mexicanchildren
Autore:
Farkas, T; Jiang, X; Guerrero, ML; Zhong, WM; Wilton, N; Berke, T; Matson, DO; Pickering, LK; Ruiz-Palacios, G;
Indirizzi:
Eastern Virginia Med Sch, Ctr Pediat Res, Norfolk, VA 23510 USA Eastern Virginia Med Sch Norfolk VA USA 23510 Res, Norfolk, VA 23510 USA Childrens Hosp Kings Daughters, Ctr Pediat Res, Norfolk, VA 23510 USA Childrens Hosp Kings Daughters Norfolk VA USA 23510 Norfolk, VA 23510 USA Inst Nutr, Dept Infect Dis, Mexico City, DF, Mexico Inst Nutr Mexico CityDF Mexico Dept Infect Dis, Mexico City, DF, Mexico
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 2, volume: 62, anno: 2000,
pagine: 217 - 223
SICI:
0146-6615(200010)62:2<217:PAGDOH>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROUND-STRUCTURED VIRUSES; NORWALK-LIKE VIRUSES; LINKED IMMUNOSORBENT ASSAYS; CAPSID PROTEIN; SOUTHEAST-ASIA; MOLECULAR EPIDEMIOLOGY; ACUTE GASTROENTERITIS; ROTAVIRUS INFECTION; ENZYME-IMMUNOASSAY; GIARDIA-LAMBLIA;
Keywords:
gastroenteritis; diarrhea; human caliciviruses; Norwalk virus; RT-PCR; sequencing; children;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Jiang, X Eastern Virginia Med Sch, Ctr Pediat Res, 855 W Brambleton Ave, Norfolk, VA 23510 USA Eastern Virginia Med Sch 855 W Brambleton Ave Norfolk VA USA 23510
Citazione:
T. Farkas et al., "Prevalence and genetic diversity of human caliciviruses (HuCVs) in Mexicanchildren", J MED VIROL, 62(2), 2000, pp. 217-223

Abstract

Human caliciviruses (HuCVs) contain two genera: "Norwalk-like viruses" (NLVs) and "Sapporo-like viruses" (SLVs). The importance of the two genera as a cause of acute gastroenteritis of infants and children remains unknown. Beginning in 1989, a birth cohort of children in Mexico was enrolled and monitored for acute gastroenteritis. A subset of 115 diarrhea stool specimens from 76 children and 66 non-diarrhea stool specimens from 64 children was examined for HuCVs by RT-PCR by using a primer pair (p289/290) that detects both NLVs and SLVs. Twenty-two (19%) of the 115 diarrhea stool specimens and 5 (7%) of 66 non-diarrhea stool specimens produced RT-PCR products of expected size (319 bp for NLVs and 331 bp for SLVs). Twenty of the twenty-seven strains were cloned and sequenced. Pairwise sequence analysis showed that9 (60%) and 6 (40%) of the 15 strains from the diarrhea stools were NLVs and SLVs, respectively. The same proportions of NLVs (60%) and SLVs (40%) were observed in the non-diarrhea stools. Strains in the NLV genus could be further divided into four clusters: Lordsdale, MxV, and HV and one potentially new cluster. Strains in the SLV genus could be divided into three clusters: Sapporo/82, Lon/92, and a potentially new cluster. Strains from the Lordsdale cluster were the most common among these children. The findings of both genera and multiple clusters of HuCVs co-circulating and the identification of new strains of HuCVs in the population justify the need for future studies of HuCVs in infants and children. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:21:57