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Titolo:
Co-receptor usage was more predictive than NSI/SI phenotype for HIV replication in macrophages: is NSI/SI phenotyping sufficient?
Autore:
Lathey, JL; Brambilla, D; Goodenow, MM; Nokta, M; Rasheed, S; Siwak, EB; Bremer, JW; Huang, DD; Yi, YJ; Reichelderfer, PS; Collman, RG;
Indirizzi:
Univ Calif San Diego, Dept Pediat, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 Pediat, La Jolla, CA 92093 USA New England Res Inst, Watertown, MA 02172 USA New England Res Inst Watertown MA USA 02172 Inst, Watertown, MA 02172 USA Univ Florida, Dept Pathol, Gainesville, FL 32611 USA Univ Florida Gainesville FL USA 32611 t Pathol, Gainesville, FL 32611 USA Univ Texas, Med Branch, Dept Internal Med, Galveston, TX 77550 USA Univ Texas Galveston TX USA 77550 t Internal Med, Galveston, TX 77550 USA Univ So Calif, Dept Pathol, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 Pathol, Los Angeles, CA 90089 USA Baylor Coll Med, Dept Otorhinolaryngol & Communicat Sci, Houston, TX 77030USA Baylor Coll Med Houston TX USA 77030 Communicat Sci, Houston, TX 77030USA Rush Med Coll, Dept Immunol Microbiol, Chicago, IL 60612 USA Rush Med Coll Chicago IL USA 60612 munol Microbiol, Chicago, IL 60612 USA Univ Penn, Pulm Allergy & Crit Care Div, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Care Div, Philadelphia, PA 19104 USA NICHHD, NIH, Bethesda, MD 20892 USA NICHHD Bethesda MD USA 20892NICHHD, NIH, Bethesda, MD 20892 USA
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 3, volume: 68, anno: 2000,
pagine: 324 - 330
SICI:
0741-5400(200009)68:3<324:CUWMPT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; TYPE-1 HIV-1; FUNCTIONAL CORECEPTOR; CHEMOKINE RECEPTORS; DISEASE PROGRESSION; CELL DECLINE; INFECTION; TRANSMISSION; VARIANTS; CXCR4;
Keywords:
macrophage tropism; viral phenotype; HIV co-receptors; HIV replication kinetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Lathey, JL Univ Calif San Diego, Dept Pediat, 9500 Gilman Dr 0672, La Jolla, CA 92093USA Univ Calif San Diego 9500 Gilman Dr 0672 La Jolla CA USA 92093
Citazione:
J.L. Lathey et al., "Co-receptor usage was more predictive than NSI/SI phenotype for HIV replication in macrophages: is NSI/SI phenotyping sufficient?", J LEUK BIOL, 68(3), 2000, pp. 324-330

Abstract

A monocyte-derived macrophage (MDM) culture assay was used to define the replication culture assay was used to define the replication kinetics of HN isolates. Ten-day-old MDMs were infected with HIV, Supernatants were collected and assayed for HIV p24 on days 3, 7, 10, and 14 post-infection (PI). In this assay, SF162 (macrophage tropic, NSI) produced increasing amounts ofHIV p24 antigen. with increasing time in culture. BRU (nonmacrophage tropic, SI) infection resulted in low levels of HIV p24 antigen with no increasein production during the culture period. A panel of 12 clinical isolates was evaluated. All isolates produced detectable levels of HIV p24 antigen inMDMs. However, the NSI viruses had significantly higher log(10) HIV p24 antigen values at all times PI (P < 0.01). Co-receptor usage was determined for all 12 isolates (8 NSI and 4 SI). All SI isolates used CXCR4 for entry; two used CXCR4 only, one used CXCR4, CCR5, and CCR3, and one was a mixture of two isolates using CXCR4 and CCR5. None of the NSI viruses used CXCR4 for entry. All used CCR5 as their predominant coreceptor. Of the eight NSI isolates, three used CCR5 only, two used CCR5 and CCR2b, one used CCR5 and CCR3, and one used CCR5, CCR3, and CCR2b, Log(10) HIV p24 antigen production on day 14 PI for viruses that used CCR5+CCR3 (3.19 + 1.40) was greater thanfor viruses that used CCR5+CCR2b (3.22 + 1.55) or CCR5 (3.32 + 1.49), and all were greater than those that used CXCR4 only (1.69 + 0.28), regardless of SI phenotype (P < 0.05). Thus, in these primary isolates, macrophage tropism and replication kinetics were closely linked to CCR5 utilization, whereas SI capacity was closely linked to CXCR4 utilization, Furthermore, viruses, which could use CCRS and CCR3 for entry, had a replication advantage inmacrophages, regardless of SI phenotype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:23:51