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Titolo:
High-performance liquid chromatographic determination of cocaine and its metabolites in serum microsamples with fluorimetric detection and its application to pharmacokinetics in rats
Autore:
Sun, L; Hall, G; Lau, CE;
Indirizzi:
Rutgers State Univ, Dept Psychol, Piscataway, NJ 08854 USA Rutgers State Univ Piscataway NJ USA 08854 chol, Piscataway, NJ 08854 USA Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA Rutgers State Univ Piscataway NJ USA 08854 Chem, Piscataway, NJ 08854 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 745, anno: 2000,
pagine: 315 - 323
SICI:
1387-2273(20000818)745:2<315:HLCDOC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ECGONINE METHYL-ESTER; ULTRAVIOLET DETECTION; PLASMA; COCAETHYLENE; BENZOYLECGONINE; NORCOCAINE; ALPRAZOLAM; MIDAZOLAM; CAFFEINE;
Keywords:
cocaine; benzoylecgonine; benzoylnorecgonine; cocaethylene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Sun, L Rutgers State Univ, Dept Psychol, 152 Frelinghuysen Rd, Piscataway,NJ 08854 USA Rutgers State Univ 152 Frelinghuysen Rd Piscataway NJ USA 08854 USA
Citazione:
L. Sun et al., "High-performance liquid chromatographic determination of cocaine and its metabolites in serum microsamples with fluorimetric detection and its application to pharmacokinetics in rats", J CHROMAT B, 745(2), 2000, pp. 315-323

Abstract

A sensitive, selective and simple HPLC method with fluorimetric detection is described for quantitating cocaine and its three metabolites in rat serum microsamples (50 mu l). Chromatographic separation is achieved on a Hypersil BDS C-18 column (100x2.1 mm, 5 mu m) with an isocratic mobile phase consisting of methanol-acetonitrile-25.8 mM sodium acetate buffer, pH 2.6, containing 1.0.10(-4) M tetrabutylammonium phosphate (14:10:76, v/v/v). The detection limit (0.5 ng/ml) for all the compounds, using direct fluorometric detection operated at excitation and emission wavelengths of 230 and 315 nm, respectively, was approximately five-times lower than that of using a UV detector operated at 235 nm. The effects of ratio of 2-propanol to chloroform in extraction solvents on the recovery and precision for cocaine and itsmetabolites were systematically examined. The method was used to study thepharmacokinetics of cocaine after administration of intravenous 2 mg/kg and oral 20 mg/kg doses. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 07:18:48