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Titolo:
Molecular cloning and characterization of another leukotriene B-4 receptor
Autore:
Kamohara, M; Takasaki, J; Matsumoto, M; Saito, T; Ohishi, T; Ishii, H; Furuichi, K;
Indirizzi:
Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Mol Med Labs, Tsukuba, Ibaraki 3058585, Japan Yamanouchi Pharmaceut Co Ltd Tsukuba Ibaraki Japan 3058585 3058585, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 35, volume: 275, anno: 2000,
pagine: 27000 - 27004
SICI:
0021-9258(20000901)275:35<27000:MCACOA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOPAMINE D4 RECEPTOR; IN-VITRO; CDNA CLONING; IDENTIFICATION; INFLAMMATION; ACTIVATION; EXPRESSION; POTENT; EOSINOPHILS; DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Kamohara, M Yamanouchi Pharmaceut Co Ltd, Inst Drug Discovery Res, Mol MedLabs, 21 Miyukigaoka, Tsukuba, Ibaraki 3058585, Japan Yamanouchi Pharmaceut Co Ltd 21 Miyukigaoka Tsukuba Ibaraki Japan 3058585
Citazione:
M. Kamohara et al., "Molecular cloning and characterization of another leukotriene B-4 receptor", J BIOL CHEM, 275(35), 2000, pp. 27000-27004

Abstract

Leukotriene B-4 is a potent lipid mediator known to be implicated mainly in inflammatory actions. Previous pharmacological studies indicated the existence of only one class of G protein-coupled receptor for leukotriene B-4, for which a candidate gene, namely BLT, had been identified. Here we reportthe isolation of another gene encoding a functional G protein-coupled receptor for leukotriene B-4, named JULF2. JULF2 is a novel G protein-coupled receptor of 358 amino acids that shares 36.6% amino acid identity with humanBLT, According to genomic information, the JULF2 gene is located on the chromosome 14, about 4 kilobases upstream of the BLT gene. During screening of endogenous ligands for JULF2, we found that leukotriene B-4 induced inhibition of forskolin-stimulated cAMP accumulation in Chinese hamster ovary cells, stably expressing JULF2, Additionally, Chinese hamster ovary cells expressing exogenous JULF2 showed chemotactic responses with leukotriene B-4 in a pertussis toxin-sensitive manner. A large amount of JULF2 mRNA was detected in the human spleen and the peripheral blood leukocytes. Furthermore, JULF2 mRNA was expressed in mononuclear lymphocytes, in which BLT mRNA was barely detected. The discovery of this second leukotriene B-4 receptor willeventually lead to a better understanding of the classification of leukotriene B-4 receptors and reconsideration of the pathophysiological role of leukotriene B-4.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 15:07:00