Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of CRAM, a novel unc-33 gene family protein that associateswith CRIMP3 and protein-tyrosine kinase(s) in the developing rat brain
Autore:
Inatome, R; Tsujimura, T; Hitomi, T; Mitsui, N; Hermann, P; Kuroda, S; Yamamura, H; Yanagi, S;
Indirizzi:
Kobe Univ, Sch Med, Dept Biochem, Chuo Ku, Kobe, Hyogo 6500017, Japan KobeUniv Kobe Hyogo Japan 6500017 m, Chuo Ku, Kobe, Hyogo 6500017, Japan Osaka Univ, Inst Sci & Ind Res, Dept Struct Mol Biol, Ibaraki, Osaka 5670047, Japan Osaka Univ Ibaraki Osaka Japan 5670047 iol, Ibaraki, Osaka 5670047, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 35, volume: 275, anno: 2000,
pagine: 27291 - 27302
SICI:
0021-9258(20000901)275:35<27291:IOCANU>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH CONE COLLAPSE; AXON GUIDANCE; SEMAPHORIN RECEPTOR; NEUROPILIN; CELLS; OUTGROWTH; NEURONS; P72(SYK); ULIP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Yanagi, S Kobe Univ, Sch Med, Dept Biochem, Chuo Ku, Kobe, Hyogo 6500017, Japan Kobe Univ Kobe Hyogo Japan 6500017 , Kobe, Hyogo 6500017, Japan
Citazione:
R. Inatome et al., "Identification of CRAM, a novel unc-33 gene family protein that associateswith CRIMP3 and protein-tyrosine kinase(s) in the developing rat brain", J BIOL CHEM, 275(35), 2000, pp. 27291-27302

Abstract

Four members of collapsin response mediator proteins (CRMPs) are thought to be involved in the semaphorin-induced growth cone collapse during neural development. Here we report the identification of a novel CRMPS associated protein, designated CRAM for CRMP3-associated molecule, that belongs to theunc-33 gene family. The deduced amino acid sequence reveals that the CRAM gene encodes a protein of 563 amino acids, shows 57% identity with dihydropyrimidinase, and shows 50-51% identity with CRMPs. CRAM appears to form a large complex composed of CRMPS and other unidentified proteins in vivo. Indeed, CRAM physically associates with CRMPS when co-expressed in COS-7 cells. The expression of CRAM is brain-specific, is high in fetal and neonatal rat brain, and decreases to very low levels in adult brain. Moreover, CRAM expression is up-regulated during neuronal differentiation of embryonal carcinoma P19 and PC12 cells. Finally, immunoprecipitation analysis of rat brain extracts shows that CRAM is co-immunoprecipitated with proteins that contain protein-tyrosine kinase activity, Taken together, our results suggest that CRAM, which interacts with CRMP3 and protein-tyrosine kinase(s), is a new member of an emerging family of molecules that potentially mediate signals involved in the guidance and outgrowth of axons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:24:11