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Titolo:
Polymerase chain reaction-based assay for antibody-mediated neutralizationof HIV-1 reveals a population of nonneutralized virus undetected by conventional p24 assay
Autore:
Achkar, JM; Wang, XH; Nyambi, P; Gorny, MK; Zolla-Pazner, S; Bandres, JC;
Indirizzi:
New York Vet Affairs Med Ctr, New York, NY 10010 USA New York Vet Affairs Med Ctr New York NY USA 10010 New York, NY 10010 USA NYU, Dept Med, New York, NY 10016 USA NYU New York NY USA 10016NYU, Dept Med, New York, NY 10016 USA NYU, Dept Pathol, New York, NY 10016 USA NYU New York NY USA 10016NYU, Dept Pathol, New York, NY 10016 USA
Titolo Testata:
JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
fascicolo: 3, volume: 24, anno: 2000,
pagine: 203 - 210
SICI:
1525-4135(20000701)24:3<203:PCRAFA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HUMAN MONOCLONAL-ANTIBODY; PBL-SCID MICE; PASSIVE-IMMUNIZATION; BINDING DOMAIN; TYPE-1 HIV-1; V3 DOMAIN; GP120; INFECTION; ENVELOPE;
Keywords:
HIV-1 neutralization; polymerase chain reaction; HIV antibodies;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Bandres, JC New York Vet Affairs Med Ctr, 4 East 23rd St,Room 1812N, New York, NY 10010 USA New York Vet Affairs Med Ctr 4 East 23rd St,Room 1812N New York NY USA 10010
Citazione:
J.M. Achkar et al., "Polymerase chain reaction-based assay for antibody-mediated neutralizationof HIV-1 reveals a population of nonneutralized virus undetected by conventional p24 assay", J ACQ IMM D, 24(3), 2000, pp. 203-210

Abstract

To be successful with strategies involving passive immunization or the generation of neutralizing antibodies against HIV, it is crucial that we improve our understanding of the process of antibody-mediated HIV neutralization. We have used a neutralization assay based on polymerase chain reaction (PCR) that is more rapid and sensitive than the conventional p24 neutralization assay based on enzyme-linked immunosorbent assay (ELISA). PCR assays permit measurement of the number of infectious events and can detect small amounts of HIV-1 only a few days postinfection. in these studies, the human anti-V3 monoclonal antibody 694/98-D was used to neutralize the infectivity of the laboratory isolate HIVIIIB for CEM-SS cells. 8E5/LAV cells, which contain a single integrated copy of proviral DNA per cell, served as a standard to determine the amount of HIV-1 copies in infected CEM-SS cells. Evaluation of antibody-mediated neutralization was possible at 2 to 3 days postinfection, at a time when p24 readouts were not conclusive. We achieved >95% neutralization of HIVIIIB, and of its molecular clone HXB2, using the monoclonal antibody 694/98-D. This degree of neutralization is probably highly significant in vivo. Nevertheless, a small amount of both HIVIIIB and HXB2 (similar to 5%) escapes neutralization and can consistently be detected aftera few days by this sensitive assay. Experiments with different anti-HIV monoclonal antibodies and viruses showed that the assay could be applied to anti-V3 as well as anti-CD4 binding domain antibodies as well as HIV laboratory strains or primary isolates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 13:12:29