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Titolo:
Cyclin-dependent kinase inhibitory protein express in human choroidal melanoma tumors
Autore:
Mouriaux, F; Maurage, CA; Labalette, P; Sablonniere, B; Malecaze, F; Darbon, JM;
Indirizzi:
Calmette Hosp, Div Pathol, Lille, France Calmette Hosp Lille FranceCalmette Hosp, Div Pathol, Lille, France Salengro Hosp, Div Biochem, Lille, France Salengro Hosp Lille FranceSalengro Hosp, Div Biochem, Lille, France Huriez Hosp, Div Ophthalmol, Lille, France Huriez Hosp Lille FranceHuriez Hosp, Div Ophthalmol, Lille, France Purpan Hosp, INSERM, CJF 95 10, Toulouse, France Purpan Hosp Toulouse France n Hosp, INSERM, CJF 95 10, Toulouse, France
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 10, volume: 41, anno: 2000,
pagine: 2837 - 2843
SICI:
0146-0404(200009)41:10<2837:CKIPEI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SQUAMOUS-CELL CARCINOMA; CUTANEOUS MALIGNANT-MELANOMA; UVEAL MELANOMA; BREAST-CANCER; DIFFERENTIAL EXPRESSION; PROGNOSTIC IMPLICATIONS; P27(KIP1) EXPRESSION; SUPPRESSOR GENE; INVASIVE STAGE; MICE LACKING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Mouriaux, F Ctr Hosp Reg & Univ Lille, Hop Huriez, Div Ophthalmol, 2 Ave OLambret, F-59037 Lille, France Ctr Hosp Reg & Univ Lille 2 Ave O Lambret Lille France F-59037
Citazione:
F. Mouriaux et al., "Cyclin-dependent kinase inhibitory protein express in human choroidal melanoma tumors", INV OPHTH V, 41(10), 2000, pp. 2837-2843

Abstract

PURPOSE. Recent studies have demonstrated the close link between oncogenesis and cell cycle machinery. Cyclin-dependent kinase inhibitory proteins (CKIs) have been shown to play a critical role in the regulation of cell cycle progression. Alteration of CKI levels and/or functions could be implicated in cell transformation. The three CKIs-p16, p21, and p27-were investigated in human uveal melanoma tumors, and an attempt was made to correlate their levels with clinicopathologic parameters, as well as to p53 and Ki-67 (Mib-1) protein levels. METHODS. Immunochemistry was performed on 32 formalin-fixed, paraffin-embedded specimens of malignant choroidal melanoma. Immunoblot was performed toconfirm the immunochemistry study. Prognostic histologic markers such as cell typing, pigmentation, larger tumor dimension, mitotic figures, nucleolar size, scleral invasion, and optic nerve head invasion were reported. RESULTS. Nuclear positivity for p16 was observed in 11 tumors (34%) without any association with clinicopathologic parameters. Tumor cells positive for p21 were detected in 12 choroidal melanomas (37%). Unexpectedly, a positive relationship was seen between p21 and scleral invasion (P = 0.008). Nuclear positivity for p27 was observed in nine tumors (28%). An inverse correlation was observed between the number of mitotic figures and p27 immunoreactivity (P = 0.03), as well as between Mib-1 positivity and p27 expression (P = 0.02). Western blot assays of tumor extracts confirmed overexpression of p21 and p27. CONCLUSIONS. The results suggest that p21 and p27 may be involved in tumorigenesis in choroidal melanoma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:21:34