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Titolo:
Proteome analysis of rat hepatic stellate cells
Autore:
Kristensen, DB; Kawada, N; Imamura, K; Miyamoto, Y; Tateno, C; Seki, S; Kuroki, T; Yoshizato, K;
Indirizzi:
Hiroshima Univ, Grad Sch Sci, Dept Biol Sci, Dev Biol Lab, Hiroshima 7398526, Japan Hiroshima Univ Hiroshima Japan 7398526 iol Lab, Hiroshima 7398526, Japan JST, Hiroshima Prefecture Joint Res Project Reg Intens, Hiroshima Tissue Regenerat Project, Hiroshima, Japan JST Hiroshima Japan iroshima Tissue Regenerat Project, Hiroshima, Japan Osaka City Univ, Sch Med, Dept Internal Med 3, Osaka 545, Japan Osaka CityUniv Osaka Japan 545 d, Dept Internal Med 3, Osaka 545, Japan
Titolo Testata:
HEPATOLOGY
fascicolo: 2, volume: 32, anno: 2000,
pagine: 268 - 277
SICI:
0270-9139(200008)32:2<268:PAORHS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMOBILIZED PH GRADIENTS; CALCIUM-BINDING PROTEIN; SMOOTH-MUSCLE-ACTIN; ALPHA-1-ANTITRYPSIN DEFICIENCY; ITO CELLS; LIVER FIBROSIS; 2-DIMENSIONAL ELECTROPHORESIS; TRANSIN STROMELYSIN; SAMPLE APPLICATION; MASS-SPECTROMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshizato, K Hiroshima Univ, Grad Sch Sci, Dept Biol Sci, Dev Biol Lab, 1-3-1 Kagamiyama, Hiroshima 7398526, Japan Hiroshima Univ 1-3-1 Kagamiyama Hiroshima Japan 7398526 apan
Citazione:
D.B. Kristensen et al., "Proteome analysis of rat hepatic stellate cells", HEPATOLOGY, 32(2), 2000, pp. 268-277

Abstract

Proteome analysis was performed on cellular and secreted proteins of normal (quiescent) and activated rat hepatic stellate cells. The stellate cells were activated either in vitro by cultivating quiescent stellate cells for 9 days or in vivo by injecting rats with carbon tetrachloride for 8 weeks. A total of 43 proteins/polypeptides were identified, which altered their expression levels when the cells were activated in vivo and/or in vitro, Twenty-seven of them showed similar changes in vivo and in vitro, including up-regulated proteins such as calcyclin, calgizzarin, and galectin-1 as well as down-regulated proteins such as liver carboxylesterase 10 and serine protease inhibitor 3, Sixteen of them showed different expression levels between in vivo and in vitro activated stellate cells. These results were reproducibly obtained in 3 independent experiments, The up-regulation of calcyclin, calgizzarin, and galectin-1, as well as the down-regulation of liver carboxylesterase 10 were directly confirmed in fibrotic liver tissues. Northernblots confirmed up-regulation of the messenger RNAs (mRNAs) of calcyclin, calgizzarin, and galectin-1 in activated stellate cells, indicating that these changes were controlled at the mRNA level. In addition a list compilingover 150 stellate cell proteins is presented. The data presented here thusprovide a significant new protein-level insight into the activation of hepatic stellate cells, a key event in liver fibrogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:34:19