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Titolo:
The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-like serine proteases
Autore:
Brown, GD; Dave, JA; van Pittius, NCG; Stevens, L; Ehlers, MRW; Beyers, AD;
Indirizzi:
Univ Stellenbosch, Dept Med Biochem, MRC, Ctr Cellular & Mol Biol, ZA-7505Tygerberg, South Africa Univ Stellenbosch Tygerberg South Africa ZA-7505 Tygerberg, South Africa Univ Cape Town, Sch Med, Dept Med Biochem, ZA-7925 Observatory, South Africa Univ Cape Town Observatory South Africa ZA-7925 bservatory, South Africa
Titolo Testata:
GENE
fascicolo: 1-2, volume: 254, anno: 2000,
pagine: 147 - 155
SICI:
0378-1119(20000822)254:1-2<147:TMOMTH>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPRESSION; SMEGMATIS; SEQUENCE; CLONING; IDENTIFICATION; TRANSFORMATION; GENERATION; GENE;
Keywords:
BCG; export; protein modification; proteolysis; transmembrane;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Brown, GD Univ Oxford, Sir William Dunn Sch Pathol, S Parks Rd, Oxford OX13RE, England Univ Oxford S Parks Rd Oxford England OX1 3RE OX1 3RE, England
Citazione:
G.D. Brown et al., "The mycosins of Mycobacterium tuberculosis H37Rv: a family of subtilisin-like serine proteases", GENE, 254(1-2), 2000, pp. 147-155

Abstract

There is little information regarding the role of proteolysis in Mycobacterium tuberculosis and no studies on the potential involvement of proteases in the pathogenesis of tuberculosis. We identified five M. tuberculosis genes (mycP1-5) that encode a family of serine proteases (mycosins-1 to 5), ranging from 36 to 47% identity. Each protein contains a catalytic triad (Asp, His, Ser) within highly conserved sequences, typical of proteases of the subtilisin family. These genes are also present in M bovis BCG and other virulent mycobacteria, but only one homologue (mycP3) was detected in M. smegmatis. The mycosins have N-terminal signal sequences and C-terminal transmembrane anchors, and the localisation of the mycosins to the membrane/cell wall was verified by Western blot analysis of heterologously expressed proteins in cellular fractions of M. smegmatis. In M. tuberculosis, all the mycosins were expressed constitutively during growth in broth. Mycosins-2 and 3were also expressed constitutively in M. bovis BCG, but no expression of mycosin-1 was detected. Mycosin-2 was modified by cleavage in all three mycobacterial species. The multiplicity and constitutive expression of these proteins suggests that they have an important role in the biology of M. tuberculosis. (C) 2000 Elsevier Science B.V. All lights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:23:48