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Titolo:
Promoter shuffling has occurred during the evolution of the vertebrate growth hormone gene
Autore:
Chuzhanova, NA; Krawczak, M; Nemytikova, LA; Gusev, VD; Cooper, DN;
Indirizzi:
Univ Wales Coll Med, Inst Med Genet, Cardiff CF14 4XN, S Glam, Wales Univ Wales Coll Med Cardiff S Glam Wales CF14 4XN CF14 4XN, S Glam, Wales Univ Wales, Dept Comp Sci, Cardiff CF24 3XF, S Glam, Wales Univ Wales Cardiff S Glam Wales CF24 3XF Cardiff CF24 3XF, S Glam, Wales Russian Acad Sci, Inst Math, Novosibirsk 630090, Russia Russian Acad Sci Novosibirsk Russia 630090 h, Novosibirsk 630090, Russia
Titolo Testata:
GENE
fascicolo: 1-2, volume: 254, anno: 2000,
pagine: 9 - 18
SICI:
0378-1119(20000822)254:1-2<9:PSHODT>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTIONAL REGULATION; BINDING-SITE; ELEMENTS; REGION; EXPRESSION; ACTIVATION; SEQUENCES; CELLS;
Keywords:
complexity analysis; evolutionary divergence; gene structure; mutation; regulatory regions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Cooper, DN Univ Wales Coll Med, Inst Med Genet, Cardiff CF14 4XN, S Glam, Wales Univ Wales Coll Med Cardiff S Glam Wales CF14 4XN Glam, Wales
Citazione:
N.A. Chuzhanova et al., "Promoter shuffling has occurred during the evolution of the vertebrate growth hormone gene", GENE, 254(1-2), 2000, pp. 9-18

Abstract

Comparative studies of vertebrate gene promoter regions seldom detect gross rearrangements ('promoter shuffling' since such analyses usually employ relatively similar DNA sequences. Conversely, attempts to compare evolutionarily more divergent promoter sequences have been largely unsuccessful owingto the inability of conventional alignment procedures to deal with gross rearrangements. These limitations have been circumvented in the present study by using the novel technique of complexity analysis to identify modular components ('blocks') in the growth hormone (GH) gene promoter sequences of some 22 vertebrate species, From salmon to human. Significant rearrangementof blocks was found to have occurred, indicating that they have evolved asindependent units. Some blocks appear to be ubiquitous, whereas others arerestricted to a specific taxon. Considerable variation between orthologousGH gene promoters was apparent in terms of block length, copy number and relative location. It may be inferred that a wide variety of different mutational mechanisms have operated upon the GH gene promoter over evolutionary time. These include gross changes such as deletion, duplication, amplification, elongation, contraction, transposition, inversion and fusion, as well as the slow, steady accumulation of single base-pair substitutions. Thus the patchwork structure of the modular GH promoter region, and those of its paralogous GH2 and prolactin (PRL) counterparts, have continually been shuffled into new combinations through the rearrangement of pre-existing blocks. Although some of these changes may have had no influence on promoter function, others could have served to alter either the level of gene expression or the responsiveness of the promoter to external stimuli. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 19:58:38