Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Immunohistochemically visualized localisation of gangliosides G(lac)2 (GD(3)) and G(tri)2 (GD(2)) in cells of human intracranial tumors
Autore:
Mennel, HD; Bosslet, K; Geissel, H; Bauer, BL;
Indirizzi:
Univ Marburg, Med Zentrum Pathol, Abt Neuropathol, D-35043 Marburg, Germany Univ Marburg Marburg Germany D-35043 uropathol, D-35043 Marburg, Germany Scheringwerke AG, Berlin, Germany Scheringwerke AG Berlin GermanyScheringwerke AG, Berlin, Germany Univ Marburg, Neurochirurg Klin, D-35032 Marburg, Germany Univ Marburg Marburg Germany D-35032 rurg Klin, D-35032 Marburg, Germany
Titolo Testata:
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
fascicolo: 4, volume: 52, anno: 2000,
pagine: 277 - 285
SICI:
0940-2993(200008)52:4<277:IVLOGG>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BRAIN TUMORS; HUMAN GLIOMAS; RAT-BRAIN; ASTROGLIA; NEURONS; GLYCOSPHINGOLIPIDS; ASTROCYTOMAS; ANTIBODIES;
Keywords:
gangliosides, intracranial tumors; intracranial tumors, gangliosides; tumors, intracranial; brain tumors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Mennel, HD Univ Marburg, Med Zentrum Pathol, Abt Neuropathol, Baldingerstr, D-35043 Marburg, Germany Univ Marburg Baldingerstr Marburg Germany D-35043 urg, Germany
Citazione:
H.D. Mennel et al., "Immunohistochemically visualized localisation of gangliosides G(lac)2 (GD(3)) and G(tri)2 (GD(2)) in cells of human intracranial tumors", EXP TOX PAT, 52(4), 2000, pp. 277-285

Abstract

Antibodies against two major gangliosides detected in human brain and brain tumors - G(lac)2 (GD(3)) and G(tri)2 (GD(2)) - were tested by immunohistochemistry in an unselected sample of intracranial tumors during the years 1986 through 1991. Two groups emerged as evaluable samples, namely gliomas of different grades and meningiomas. In a pilot series, it was shown that these gangliosides could be visualized in frozen sections of cells of gliomasand meningiomas (as well as neurinomas) and in some structures of the normal brain. It was however not possible in frozen sections to further analyze the cellular or subcellular expression pattern of the mentioned components and paraffin sections with conventional processing were: only weakly and diffusely stained. A modified protocol therefore was created that replaced alcohol processing by acetone. With this protocol, interpretable results in paraffin sections were obtained. With this method, 133 single intracranial tumors were investigated as to their immunohistologically detectable ganglioside expression. The most consistent result was that the whole cytoplasm of gliomas was stained by G(tri)2 (GD(2)) and G(lac)2 (GD(3)) with high preponderance of G(tri)2 (GD(2)) expression. In all meningiomas, G(lac)2 (GD(3)) had a higher expression than G(tri)2. No constant pattern in the other entities emerged. By comparison with GFAP expression in gliomas and vimentin in meningiomas, the colocalisation of gangliosides and intermediary filament proteins is supposed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 22:47:05