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Titolo:
Zinc sulphate induces metallothionein in pancreatic islets of mice and protects against diabetes induced by multiple low doses of streptozotocin
Autore:
Ohly, P; Dohle, C; Abel, J; Seissler, J; Gleichmann, H;
Indirizzi:
Univ Dusseldorf, German Diabet Res Inst, German Diabet Ctr, D-4000 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-4000 tr, D-4000 Dusseldorf, Germany Univ Dusseldorf, Med Inst Environm Hyg, Div Toxicol, D-4000 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-4000 ol, D-4000 Dusseldorf, Germany
Titolo Testata:
DIABETOLOGIA
fascicolo: 8, volume: 43, anno: 2000,
pagine: 1020 - 1030
SICI:
0012-186X(200008)43:8<1020:ZSIMIP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE GENERATION; OXIDATIVE STRESS; FREE-RADICALS; GENE-EXPRESSION; HUMAN-DISEASE; BETA-CELLS; IN-VITRO; SUPEROXIDE; INDUCTION; DESTRUCTION;
Keywords:
streptozotocin; metallothionein; zinc sulphate; diabetes; beta cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Ohly, P Deutsch Diabet Forschungsinst, Hennekamp 65, D-40225 Dusseldorf, Germany Deutsch Diabet Forschungsinst Hennekamp 65 Dusseldorf Germany D-40225
Citazione:
P. Ohly et al., "Zinc sulphate induces metallothionein in pancreatic islets of mice and protects against diabetes induced by multiple low doses of streptozotocin", DIABETOLOG, 43(8), 2000, pp. 1020-1030

Abstract

Aims/hypothesis. Diabetes is induced by multiple low closes of streptozotocin (MLD-STZ) in male mice of susceptible strains. In this model beta-cell injury and T-cell-mediated inflammatory reactions are induced. probably reactive oxygen species (ROS) participate in the destruction of beta cells. The effects of ROS can be counterbalanced by several antioxidant systems. Oneof these is metallothionein (MT), cytosolic proteins that are induced by zinc ions (Zn2+) and scavenge hydroxyl radicals ((OH)-O-.). The effect of Zn2+ on MLD-STZ-diabetes was studied. Methods. We gave C57BL/6 and (C57BL/6 x SJL)F-1 hybrid mice either MLD-STZor in addition Zn2+-enriched drinking water. We analysed metallothionein ex vivo in pancreatic islets for protein and mRNA concentration for the isoforms 1 and 2. Pancreatic sections were examined by immunohistochemistry formetallothionein and histologically for insulitis. Results. In both strains, Zn2+-enriched drinking water significantly up-regulated metallothionein and prevented MLD-STZ-diabetes and loss of beta-cell function. In the F-1 hybrid mice a variant of MLD-STZ-diabetes was observed. These mice developed hyperglycaemia 10 weeks after the first injection of STZ (in contrast to 2 weeks observed in other mouse strains) and pronounced insulitis, The mRNA of the metallothionein isoforms 1 and 2 were constitutively expressed and slightly up-regulated by Zn2+-enriched drinking water, All islets cells stained for metallothionein. Conclusions/interpretations. Drinking water enriched with Zn2+ significantly up-regulated metallothionein production in pancreatic islets of mice andprevented diabetes induced with MLD-STZ.

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Documento generato il 03/12/20 alle ore 15:52:38