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Titolo:
GTP cyclohydrolase I from Tetrahymena pyriformis: cloning of cDNA and expression
Autore:
Tazawa, M; Ohtsuki, M; Sumi-Ichinose, C; Shiraishi, H; Kuroda, R; Hagino, Y; Nakashima, S; Nozawa, Y; Ichinose, H; Nagatsu, T; Nomura, T;
Indirizzi:
Fujita Hlth Univ, Sch Med, Dept Pharmacol, Aichi 4701192, Japan Fujita Hlth Univ Aichi Japan 4701192 ept Pharmacol, Aichi 4701192, Japan Gifu Univ, Sch Med, Dept Biochem, Gifu 5008705, Japan Gifu Univ Gifu Japan 5008705 Sch Med, Dept Biochem, Gifu 5008705, Japan Fujita Hlth Univ, Inst Comprehens Med Sci, Div Mol Genet, Aichi 4701192, Japan Fujita Hlth Univ Aichi Japan 4701192 Div Mol Genet, Aichi 4701192, Japan
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 1, volume: 127, anno: 2000,
pagine: 65 - 73
SICI:
0305-0491(200009)127:1<65:GCIFTP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; DICTYOSTELIUM-DISCOIDEUM; DROSOPHILA-MELANOGASTER; MOLECULAR-CLONING; ESCHERICHIA-COLI; MESSENGER-RNA; BIOSYNTHESIS; PURIFICATION; LIVER; PATHWAY;
Keywords:
cDNA cloning; CTP cyclohydrolase I; Tetrahymena pyriformis; rnRNA expression; pteridine metabolism; tetrahydrobiopterin; biopterin; neopterin; synchronous cell division; protozoa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Nomura, T Fujita Hlth Univ, Sch Med, Dept Pharmacol, Aichi 4701192, Japan Fujita Hlth Univ Aichi Japan 4701192 col, Aichi 4701192, Japan
Citazione:
M. Tazawa et al., "GTP cyclohydrolase I from Tetrahymena pyriformis: cloning of cDNA and expression", COMP BIOC B, 127(1), 2000, pp. 65-73

Abstract

A full-length cDNA clone for GTP cyclohydrolase I (EC 3.5.4.16) was isolated from a Tetrahymena pyriformis cDNA library by plaque hybridization. The nucleotide sequence determination revealed that the length of the cDNA insert was 1516 bp. The coding region encoded a protein of 223 amino acid residues with a calculated molecular mass of 25 416 Da. The deduced amino acid sequence of Tetrahymena GTP cyclohydrolase I showed sequence identity with that of Escherichia coli (55%). The identity of T. pyriformis GTP cyclohydrolase I with sequences of Dictyostelium discoideum, Saccharomyces cerevisiae, Drosophila melangaster, mouse, rat, and human enzymes was less marked andwas 30, 30, 25, 28, 28, and 27%, respectively. RNA blot analysis showed a single mRNA species of 2.1 kb in this protozoan. The mRNA level of GTP cyclohydrolase I increased during synchronous cell division induced by intermittent heat treatment. The results suggest that the mRNA expression is associated with the cell cycle of T. pyriformis. (C) 2000 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 07:26:24