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Titolo:
Discrimination of prohibited oral use of salbutamol from authorized inhaled asthma treatment
Autore:
Berges, R; Segura, J; Ventura, R; Fitch, KD; Morton, AR; Farre, M; Mas, M; de la Torre, X;
Indirizzi:
Inst Municipal Invest Med, Pharmacol Res Unit, E-08003 Barcelona, Spain Inst Municipal Invest Med Barcelona Spain E-08003 08003 Barcelona, Spain Univ Western Australia, Dept Human Movement & Exercise Sci, Perth, WA 6907, Australia Univ Western Australia Perth WA Australia 6907 Perth, WA 6907, Australia Univ Pompeu Fabra, Barcelona 08005, Spain Univ Pompeu Fabra Barcelona Spain 08005 eu Fabra, Barcelona 08005, Spain Univ Autonoma Barcelona, E-08193 Barcelona, Spain Univ Autonoma BarcelonaBarcelona Spain E-08193 E-08193 Barcelona, Spain
Titolo Testata:
CLINICAL CHEMISTRY
fascicolo: 9, volume: 46, anno: 2000,
pagine: 1365 - 1375
SICI:
0009-9147(200009)46:9<1365:DOPOUO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENANTIOSELECTIVE DISPOSITION; SULFATE CONJUGATION; MASS-SPECTROMETRY; INORGANIC SULFATE; ALBUTEROL; PHARMACOKINETICS; HUMANS; BIOAVAILABILITY; ACETAMINOPHEN; ENANTIOMERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Segura, J Inst Municipal Invest Med, Pharmacol Res Unit, Doctor Aiguader 80, E-08003Barcelona, Spain Inst Municipal Invest Med Doctor Aiguader 80 Barcelona Spain E-08003
Citazione:
R. Berges et al., "Discrimination of prohibited oral use of salbutamol from authorized inhaled asthma treatment", CLIN CHEM, 46(9), 2000, pp. 1365-1375

Abstract

Background: The administration of salbutamol is permitted only by inhalation by the international Olympic Committee (IOC) for the management of asthma and exercise-induced asthma in athletes. The establishment of criteria todistinguish between the IOC authorized use (inhaled) and the IOC prohibited use (oral) of salbutamol appeared possible using simultaneous evaluation of variables based on the concentration of nonconjugated enantiomers of salbutamol excreted in urine. Methods: Urine was collected from asthmatic and nonasthmatic swimmers who had received various preexercise doses of oral (five doses of 4 mg) or inhaled (two doses of 100 mu g) salbutamol. Urine was also obtained from subjects who had received the maximum dosage of inhaled salbutamol advisable for competing athletes to provide protection from exercise-induced asthma and treatment of asthma (1600 mu g in 24 h, 800 mu g being in the last 4 h), Allsamples were analyzed to determine the total amount of unchanged salbutamol excreted in urine and the ratio between the S and R enantiomers. Results: The discriminant function D = -3.776 + 1.46 x 10(-3) {[S(+)] + [R(-)]} + 1.012 {[S(+)]/[R(-)]} can be used to classify data into two groups,inhaled and oral. The confirmatory criterion suggested (cutoff at D = 1.06, 4 SD from the mean D value of the inhaled distribution) has been verifiedin different sets of samples showing suspicious concentrations by conventional screening procedures in doping control. An 11.8% false-negative (oral classified as inhaled) rate is assumed with the confirmatory criterion proposed, but virtually no false positives (inhaled classified as oral) are obtained (<1 in 33 000). Conclusions: The overall procedure recommended is to screen all samples and to apply the confirmation criterion proposed to samples showing free racemic salbutamol concentrations >500 9 mu g/L by gas chromatography-mass spectrometry or free + conjugated racemic salbutamol concentrations >1400 mu g/L by ELISA. (C) 2000 American Association for Clinical Chemistry.

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Documento generato il 01/04/20 alle ore 01:33:27