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Titolo:
RCC1 AND NUCLEAR-ORGANIZATION
Autore:
HUANG S; MAYEDA A; KRAINER AR; SPECTOR DL;
Indirizzi:
COLD SPRING HARBOR LAB,1 BUNGTOWN RD COLD SPRING HARBOR NY 11724 COLD SPRING HARBOR LAB COLD SPRING HARBOR NY 11724
Titolo Testata:
Molecular biology of the cell
fascicolo: 6, volume: 8, anno: 1997,
pagine: 1143 - 1157
SICI:
1059-1524(1997)8:6<1143:RAN>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREMATURE CHROMOSOME CONDENSATION; MESSENGER-RNA; CELL-CYCLE; PROTEIN IMPORT; FISSION YEAST; TRANSCRIPTION INITIATION; RCC1-RELATED PROTEIN; NASCENT TRANSCRIPTS; SPLICING INVITRO; BINDING PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
S. Huang et al., "RCC1 AND NUCLEAR-ORGANIZATION", Molecular biology of the cell, 8(6), 1997, pp. 1143-1157

Abstract

We have examined the effect of RCC1 function on the nuclear organization of pre-mRNA splicing factors and poly(A)(+) RNA in the tsBN2 cells, a RCC1 temperature-sensitive mutant cell line. We have found that at4-6 h after shifting cells from the permissive temperature (32.5 degrees C) to the restrictive temperature (39.5 degrees C), both small nuclear ribonucleoprotein particles and a general splicing factor SC35 reorganized into 4-10 large round clusters in the nucleus, as compared with the typical speckled distribution seen in cells at the permissive temperature. In situ hybridization to poly(A)(+) RNA resulted in a similar pattern. Examination by double labeling demonstrated that the redistribution of splicing factors coincides with that of poly(A)(+) RNA. Such changes in the nuclear organization of splicing factors and poly(A)(+) RNA were not the result of the temperature shift or of chromatin condensation. Cellular transcription was not significantly altered in these cells and extracts made from both the permissive and restrictive temperature were splicing competent. Electron microscopic examination demonstrated that the large clusters containing both splicing factors and poly(A)(+) RNA were fused interchromatin granule clusters. In addition, small electron-dense dot-like structures measuring approximately 80 nm in diameter were also observed, most of which are accumulated in enlarged interchromatin granule clusters in the nucleoplasm of RCC1- cells. In spite of the significant changes observed in the nucleoplasm, relatively little alteration was observed in nucleolar structureby both light and electron microscopic examination. The above observations suggest that the RCC1 protein directly or indirectly regulates the organization of splicing components and poly(A)(+) RNA in the cell nucleus and that RCC1 may play a role in nuclear organization.

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Documento generato il 25/11/20 alle ore 18:45:59