Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The pH-dependent interaction of cinnamomin with lipid membranes investigated by fluorescence methods
Autore:
Hu, RG; Tang, S; Liu, WY;
Indirizzi:
Acad Sinica, Shanghai Inst Biochem, Shanghai 20031, Peoples R China Acad Sinica Shanghai Peoples R China 20031 anghai 20031, Peoples R China
Titolo Testata:
BIOLOGICAL CHEMISTRY
fascicolo: 7, volume: 381, anno: 2000,
pagine: 567 - 573
SICI:
1431-6730(200007)381:7<567:TPIOCW>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOME-INACTIVATING PROTEINS; NECROSIS-FACTOR-ALPHA; DIPHTHERIA-TOXIN; ENERGY-TRANSFER; FRAGMENT-B; VESICLES; FUSION; SARCIN; MECHANISM; CHANNEL;
Keywords:
calcein; cinnamomin; fluorescence quenching; fusion; liposome; ribosome-inactivating protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, WY Acad Sinica, Shanghai Inst Biochem, 320 Yue Yang Rd, Shanghai 20031, Peoples R China Acad Sinica 320 Yue Yang Rd Shanghai Peoples R China 20031 China
Citazione:
R.G. Hu et al., "The pH-dependent interaction of cinnamomin with lipid membranes investigated by fluorescence methods", BIOL CHEM, 381(7), 2000, pp. 567-573

Abstract

Cinnamomin, a new type II ribosome-inactivating protein (RIP), was found to be able to induce the release of calcein loaded in lecithin small unilamellar vesicles and the fusion or aggregation of the lecithin liposomes. Suchinduction could be promoted several fold by a pH 5.0 environment, a condition similar to that in endocytic vesicles. Lowering the pH from 7.5 to 5.0 evoked conformational changes of cinnamomin and unmasked its hydrophobic areas, including the exposure of 1-anilino-8-naphthalenesulfonate (1,8-ANS) binding sites of the molecule. Some tryptophan residues with affinity to acrylamide were demonstrated to participate in the lipid-protein interaction. The pH dependent fusogenicity of type II RIP might suggest its in vivo function as a fusogen to exert its cytotoxicity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:26:16