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Titolo:
Use of the polymerase chain reaction assay for the detection of Mycobacterium avium subspecies paratuberculosis in blood and liver biopsies from experimentally infected sheep
Autore:
Gwozdz, JM; Thompson, KG; Murray, A; West, DM; Manktelow, BW;
Indirizzi:
Massey Univ, Inst Vet Anim & Biomed Sci, Palmerston North, New Zealand Massey Univ Palmerston North New Zealand Palmerston North, New Zealand
Titolo Testata:
AUSTRALIAN VETERINARY JOURNAL
fascicolo: 9, volume: 78, anno: 2000,
pagine: 622 - 624
SICI:
0005-0423(200009)78:9<622:UOTPCR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
JOHNES DISEASE; DNA-EXTRACTION; FECAL CULTURE; PCR; CATTLE; TESTS;
Keywords:
sheep; paratuberculosis; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Gwozdz, JM Victorian Inst Anim Sci, 475 Mickleham Rd, Attwood, Vic 3049, Australia Victorian Inst Anim Sci 475 Mickleham Rd Attwood Vic Australia 3049
Citazione:
J.M. Gwozdz et al., "Use of the polymerase chain reaction assay for the detection of Mycobacterium avium subspecies paratuberculosis in blood and liver biopsies from experimentally infected sheep", AUST VET J, 78(9), 2000, pp. 622-624

Abstract

Objective To evaluate a polymerase chain reaction-based assay for the detection of Mycobacterium avium subsp paratuberculosis in blood and liver biopsies from subclinically infected sheep. Study design A direct PCR assay for the detection of M a paratuberculosis was applied to liver biopsy specimens and to samples of blood that were sequentially collected over 53 weeks from 14 sheep infected experimentally with the organism. Results Of 117 blood samples from the 14 experimentally infected sheep, two tested positive in the PCR assay. Both positive results were obtained in two subclinically infected sheep that had paratuberculosis later confirmed by histological examination at necropsy. However, the assay failed to detect the target DNA in samples of blood from five other sheep with histologically confirmed paratuberculosis. Similarly, the PCR assay on liver biopsy specimens collected 32 weeks after administration of M a paratuberculosis gave only two positive results, both of which were obtained in sheep with histological evidence of paratuberculosis. Conclusions The PCR assay on blood and liver biopsies does not provide a useful method for the diagnosis of M a paratuberculosis infection in subclinically infected sheep.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:41:45