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Titolo:
Seasonal and spatial community dynamics in the meromictic Lake Cadagno
Autore:
Bosshard, PP; Stettler, R; Bachofen, R;
Indirizzi:
Univ Zurich, Inst Plant Biol, Dept Microbiol, CH-8008 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8008 biol, CH-8008 Zurich, Switzerland
Titolo Testata:
ARCHIVES OF MICROBIOLOGY
fascicolo: 3, volume: 174, anno: 2000,
pagine: 168 - 174
SICI:
0302-8933(200009)174:3<168:SASCDI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRADIENT GEL-ELECTROPHORESIS; 16S RIBOSOMAL-RNA; PHOTOTROPHIC SULFUR BACTERIA; POPULATIONS; BACTERIOPLANKTON; ENVIRONMENT; SWITZERLAND; SEDIMENT; GENES;
Keywords:
16S rDNA; temporal temperature gradient gel electrophoresis; fluorescent in situ hybridization; population dynamics; meromixis; bacterioplankton; chemocline; bacterial diversity; Chromatiaceae; purple sulfur bacteria;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Bosshard, PP Univ Zurich, Inst Plant Biol, Dept Microbiol, Zollikerstr 107, CH-8008 Zurich, Switzerland Univ Zurich Zollikerstr 107 Zurich Switzerland CH-8008 rland
Citazione:
P.P. Bosshard et al., "Seasonal and spatial community dynamics in the meromictic Lake Cadagno", ARCH MICROB, 174(3), 2000, pp. 168-174

Abstract

The seasonal and spatial variations in the community structure of bacterioplankton in the meromictic alpine Lake Cadagno were examined by temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Two different amplifications were performed. one specific for the domain Bacteria (Escherichia coli positions 8-536) and another specific for the family Chromatiaceae (E. coli positions 8-1005). The latter was followed by semi-nested reamplification with the bacterial primer set, allowing comparison of the two PCR approaches by TTGE. The TTGE patterns of samples from the chemocline and the anoxic monimolimnion were essentially identical, whereas the oxic mixolimnion displayed distinctively different banding patterns. For samples from the chemocline and the monimolimnion. dominant bandsin the Bacteria-specific TTGE profiles comigrated with bands obtained by the semi-nested PCR approach specific for Chromatiaceae. This observation suggested that Chromatiaceae are in high abundance in the anoxic water layer. All dominant bands were excised and sequenced. Changes in the community structure, as indicated by changes in the TTGE profiles. were observed in samples taken at different times of the year. In the chemocline, Chromatium okenii was dominant in the summer months, whereas Amoebobacter purpureus populations dominated in autumn and winter. This change was confirmed by fluorescent in situ hybridization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:02:44