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Titolo:
Decreased constitutive nitric oxide synthase, but increased inducible nitric oxide synthase and endothelin-1 immunoreactivity in aortic endothelial cells of Donryu rats on a cholesterol-enriched diet
Autore:
Aliev, G; Shi, J; Perry, G; Friedland, RP; Lamanna, JC;
Indirizzi:
Case Western Reserve Univ, Sch Med, Dept Neurol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Electron Microscopy Lab, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Div Neuropathol, Inst Pathol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Dept Neurol, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Dept Physiol & Biophys, Cleveland, OH 44106 USACase Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Case Western Reserve Univ, Dept Anat & Neurosci, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
ANATOMICAL RECORD
fascicolo: 1, volume: 260, anno: 2000,
pagine: 16 - 25
SICI:
0003-276X(20000901)260:1<16:DCNOSB>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HERITABLE HYPERLIPIDEMIC RABBITS; DEPENDENT RELAXATION; THORACIC AORTA; YOSHIDA RATS; HYPERCHOLESTEROLEMIC RABBITS; INDUCED ATHEROSCLEROSIS; VASCULAR REACTIVITY; SUPPLEMENTED DIET; CORONARY-ARTERIES; L-ARGININE;
Keywords:
nitric oxide synthase type I, II, and III; ET-1; atherosclerosis; cholesterol-supplemented diet; Donryu rats; endothelium; electron microscopy; immunocytochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Lamanna, JC Case Western Reserve Univ, Sch Med, Dept Neurol, 10900 Euclid Ave, Cleveland, OH 44106 USA Case Western Reserve Univ 10900 Euclid Ave Cleveland OH USA 44106
Citazione:
G. Aliev et al., "Decreased constitutive nitric oxide synthase, but increased inducible nitric oxide synthase and endothelin-1 immunoreactivity in aortic endothelial cells of Donryu rats on a cholesterol-enriched diet", ANAT REC, 260(1), 2000, pp. 16-25

Abstract

The Donryu rat is resistant to a high cholesterol diet in that typical atheromatous lesions do not develop. Using electron microscopic immunocytochemical techniques, the effects of a CCT diet (4% cholesterol with 1% cholic acid and 0.5% thiouracil) on the distributions of neuronal, macrophage, and endothelial specific nitric oxide synthase (NOS I, NOS II, and NOS III) andendothelin-1 (ET-1) immunoreactivity were examined in the thoracic aortic intima. Atheromatous lesions were absent, but immunocytochemistry showed 1.4+/-0.52% and 4.0+/-0.9% endothelial cells (EC) with positive staining for NOS I and NOS III, respectively, compared with 16.3+/-2.5% and 88.6+/-2.48%in control Donryu rats. The CCT-supplemented diet induced expression of NOS II immunoreactivity in thoracic aortic intimal cells. EC, subendothelial macrophages, and smooth muscle cells (SMC) also showed high NOS II-positivestaining. The percentage of NOS II-immunoreactive EC was 43+/-1.8%. In control groups, no NOS II immunoreactive cells were observed. The percentage of ET-1 immunopositive cells was also significantly increased by 9.2+/-0.66%and 64.2+/-1.4% in control and CCT-fed groups, respectively. It is concluded that the administration of a high cholesterol diet in Donryu rats produces endothelial dysfunction associated with changes in the balance of the different isoforms of NOS and ET-1. Therefore, the increase in inducible NOS and ET-1 immunoreactivity seen during the cholesterol-enriched diet appearsto be a compensatory reaction of aortic wall cells to the high cholesterolsupplementation. Anat Rec 260:16-25, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:26:00