Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cloning of porcine intercellular adhesion molecule-1 and characterization of its induction on endothelial cells by cytokines
Autore:
Stocker, CJ; Sugars, KL; Yarwood, H; Delikouras, A; Lechler, RI; Dorling, A; Landis, RC; Morley, BJ; Haskard, DO;
Indirizzi:
Hammersmith Hosp, Imperial Coll Sch Med, BHF Cardiovasc Med Unit, NHLI, London W12 0NN, England Hammersmith Hosp London England W12 0NN t, NHLI, London W12 0NN, England Hammersmith Hosp, Imperial Coll Sch Med, Dept Med, London W12 0NN, EnglandHammersmith Hosp London England W12 0NN ept Med, London W12 0NN, England
Titolo Testata:
TRANSPLANTATION
fascicolo: 4, volume: 70, anno: 2000,
pagine: 579 - 586
SICI:
0041-1337(20000827)70:4<579:COPIAM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; HUMAN T-CELLS; MONOCLONAL-ANTIBODY; ICAM-1 CD54; CARDIAC ALLOGRAFTS; DIRECT RECOGNITION; ANTIGEN-1 LFA-1; IFN-GAMMA; EXPRESSION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Haskard, DO Hammersmith Hosp, Imperial Coll Sch Med, BHF Cardiovasc Med Unit, NHLI, London W12 0NN, England Hammersmith Hosp London England W12 0NNdon W12 0NN, England
Citazione:
C.J. Stocker et al., "Cloning of porcine intercellular adhesion molecule-1 and characterization of its induction on endothelial cells by cytokines", TRANSPLANT, 70(4), 2000, pp. 579-586

Abstract

Background The transplantation of pig organs into humans requires a detailed knowledge of similarities and differences between the two species in themolecular physiology of host defense mechanisms. We therefore set out to identify porcine intercellular adhesion molecule (ICAM)-1 and to characterize its expression by endothelial cells. Methods. Porcine ICAM-1 cDNA was isolated from an endothelial cell cDNA library. An anti-pig ICAM-1 monoclonal antibody was generated and used to investigate the regulation by cytokines of ICAM-1 expression by porcine aorticendothelial cells (PAEC), using flow cytometry. Results. We found that porcine ICAM-1 was similar in primary structure to human. ICAM-1, with five Ig-like domains. COS-7 cells transfected with porcine ICAM-1 supported beta(2) but not alpha(4) integrin-dependent adhesion of human T lymphoblasts. There was a low-level surface expression of ICAM-1 on unstimulated PAEC and increased expression after stimulation with tumor necrosis factor (TNF)-alpha. However expression of ICAM-1 seemed to be significantly lower than that of vascular cell adhesion molecule-1, both on unstimulated and TNF-alpha-activated PAEC. Recombinant porcine interferon-gamma weakly stimulated ICAM-1 expression when incubated alone with PAEC but had an. inhibitory effect on the increase in ICAM-1 due to TNF-alpha, both at8 and 24 hr. Conclusions. Our observations confirm the existence of ICAM-1 in the pig and provide novel insights into how porcine and human endothelial cells differ in terms of adhesion molecule expression and cytokine responsiveness. Such differences are potentially important in interpreting models of inflammation in the pig and also in understanding the process of rejection of porcine xenografts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 02:49:58