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Titolo:
A dominant negative mutant of Sar1 GTPase inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus in tobacco and Arabidopsiscultured cells
Autore:
Takeuchi, M; Ueda, T; Sato, K; Abe, H; Nagata, T; Nakano, A;
Indirizzi:
RIKEN, Mol Membrane Biol Lab, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 e Biol Lab, Wako, Saitama 3510198, Japan Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Biol Sci, Bunkyo Ku, Tokyo 1130033, Japan
Titolo Testata:
PLANT JOURNAL
fascicolo: 4, volume: 23, anno: 2000,
pagine: 517 - 525
SICI:
0960-7412(200008)23:4<517:ADNMOS>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GDP DISSOCIATION INHIBITOR; BINDING PROTEIN; VESICULAR TRANSPORT; SECRETORY PATHWAY; YEAST; MEMBRANE; PLANTS; ER; COMPLEMENTATION; LOCALIZATION;
Keywords:
Sar1 GTPase; ER-to-Golgi transport; dominant mutation; vesicular traffic;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Nakano, A RIKEN, Mol Membrane Biol Lab, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 , Wako, Saitama 3510198, Japan
Citazione:
M. Takeuchi et al., "A dominant negative mutant of Sar1 GTPase inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus in tobacco and Arabidopsiscultured cells", PLANT J, 23(4), 2000, pp. 517-525

Abstract

Protein secretion plays an important role in plant cells as it does in animal and yeast cells, but the tools to study molecular events of plant secretion are very limited. We have focused on the Sari GTPase, which is essential for the vesicle formation from the endoplasmic reticulum (ER) in yeast, and have previously shown that tobacco and Arabidopsis SARI complement yeast sari mutants. In this study, we have established a transient expression system of GFP-fusion proteins in tobacco and Arabidopsis cultured cells. By utilizing confocal laser scanning microscopy, we demonstrate that a dominant negative mutant of Arabidopsis Sari inhibits the ER-to-Golgi transport ofGolgi membrane proteins, AtErd2 and AtRer1B, and locates them to the ER. The same mutant Sari also blocks the exit from the ER of a vacuolar storage protein, sporamin. These results not only provide the first evidence that the Sari GTPase functions in the ER-to-Golgi transport in plant cells, but also prove that conditional expression of dominant mutants of secretory machinery can be a useful tool in manipulating vesicular trafficking.

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Documento generato il 26/01/21 alle ore 04:26:20