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Titolo:
THE USE OF ANTIDESMOGLEIN STAINS IN MOHS MICROGRAPHIC SURGERY - A POTENTIAL AID FOR THE DIFFERENTIATION OF BASAL-CELL CARCINOMA FROM HORIZONTAL SECTIONS OF THE HAIR FOLLICLE AND FOLLICULOCENTRIC BASALOID PROLIFERATION
Autore:
KRUNIC AL; GARROD DR; VIEHMAN GE; MADANI S; BUCHANAN MD; CLARK RE;
Indirizzi:
DUKE UNIV,MED CTR,DEPT MED,DIV DERMATOL,DERMATOL SURG UNIT,BOX 3915 DURHAM NC 27710 UNIV MANCHESTER,SCH BIOL SCI,EPITHELIAL MORPHOGENESIS RES GRP MANCHESTER LANCS ENGLAND
Titolo Testata:
Dermatologic surgery
fascicolo: 6, volume: 23, anno: 1997,
pagine: 463 - 468
SICI:
1076-0512(1997)23:6<463:TUOASI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERCELLULAR-JUNCTIONS; DESMOSOMES; GLYCOPROTEIN; EPITHELIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
A.L. Krunic et al., "THE USE OF ANTIDESMOGLEIN STAINS IN MOHS MICROGRAPHIC SURGERY - A POTENTIAL AID FOR THE DIFFERENTIATION OF BASAL-CELL CARCINOMA FROM HORIZONTAL SECTIONS OF THE HAIR FOLLICLE AND FOLLICULOCENTRIC BASALOID PROLIFERATION", Dermatologic surgery, 23(6), 1997, pp. 463-468

Abstract

BACKGROUND. Histopathologic differentiation between benign and malignant tissue is of utmost importance for the Mohs surgeon. Folliculocentric basaloid proliferation (FBP) shares many histologic features with basal cell carcinoma (BCC). It is most commonly associated with tumorsof areas with abundant hair follicles such as nasal and perinasal skin. Residual BCC incorrectly identified as a horizontally sectioned hair follicle undoubtedly increases the risk of tumor recurrence. Excision of additional layers of normal tissue to remove ''funny looking follicles'' may have profound impacts on tissue conservation preservation of function; and cosmesis. Electron microscope studies of BCC revealeda significant reduction of desmosomes compared with normal basal cells and hair follicle keratinocytes. OBJECTIVE. This study has assessed the potential of rapid staining with monoclonal antidesmoglein antibody (33-3D) to discriminate between BCC, horizontally sectioned hair follicles, and FBP. METHODS. A rapid immunoperoxidase technique with 33-3D antidesmoglein antibody was performed on Mohs frozen sections. We selected 28 patients with BCC of nasal and perinasal locations where histologic discrimination between residual tumor and tumor-free margins with FBP or horizontally sectioned hair follicle was equivocal. RESULTS. Fourteen sections disclosed the preservation of desmoglein marker delineating the cell membranes (''perimembranous'' pattern) consistent with normal hair follicles. The sections were identified as tumor-free and no additional stages were performed. The remaining four sections revealed absent perimembranous pattern but presence of diffuse cytoplasmic staining. These were diagnosed as positive for residual BCC requiring the excision of another layer of tissue to obtain tumor-free margins. A follow-up period ranging from 6 to 24 months revealed no instance of recurrent disease. CONCLUSION. Rapid detection of desmoglein with33-3D antibody is a promising tool for discrimination between residual BCC and FBP or horizontally sectioned hair follicles. It may enhancethe sensitivity of Mohs surgery by disclosing the hidden foci of BCC,thus preventing tumor recurrence and unnecessary excision of normal tissue. (C) 1997 by the American Society for Dermatologic Surgery, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:51:24