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Titolo:
Structure-function relationships of two closely related group IC3 intron ribozymes from Azoarcus and Synechococcus pre-tRNA
Autore:
Ikawa, Y; Naito, D; Shiraishi, H; Inoue, T;
Indirizzi:
Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Grad Sch Biostudies, Kyoto 6068502, Japan
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 17, volume: 28, anno: 2000,
pagine: 3269 - 3277
SICI:
0305-1048(20000901)28:17<3269:SROTCR>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-I INTRON; COMPARATIVE SEQUENCE-ANALYSIS; SELF-SPLICING INTRONS; TRANSFER-RNA GENES; 2 DOMAINS; BACTERIA; REQUIREMENTS; ORIGIN; MOTIF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, T Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto UnivKyoto Japan 6068502 Biostudies, Kyoto 6068502, Japan
Citazione:
Y. Ikawa et al., "Structure-function relationships of two closely related group IC3 intron ribozymes from Azoarcus and Synechococcus pre-tRNA", NUCL ACID R, 28(17), 2000, pp. 3269-3277

Abstract

The two group IC3 pre-tRNA introns from Azoarcus and Synechococcus share very analogous secondary structures. They are small group I ribozymes that possess only two peripheral domains, P2 and P9, However, the 3'-splice site hydrolysis activity of the Synechococcus ribozyme critically depends on P2 whereas that of Azoarcus does not, indicating that the structure-function relationships of the two ribozymes are strikingly different despite their structural resemblance. To identify the element(s) that determines the catalytic properties of these ribozymes, we undertook analyses of chimeric ribozymes prepared by swapping their structural elements. We found that the difference can be attributed to a small number of nucleotides within the conserved core region. Further analysis by employing in vitro selection revealed that a base triple interaction (P4bp3 x J6/7-2) is a critical element for determining activity and suggests the existence of a novel base quintuple involving the base triple P4bp5 x J8/7-5.

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Documento generato il 22/01/20 alle ore 13:00:28