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Titolo:
Mesoaccumbens dopamine neuron synapses reconstructed in vitro are glutamatergic
Autore:
Joyce, MP; Rayport, S;
Indirizzi:
Columbia Univ, Dept Psychiat, New York, NY 10032 USA Columbia Univ New York NY USA 10032 Dept Psychiat, New York, NY 10032 USA Columbia Univ, Dept Anat & Cell Biol, New York, NY 10032 USA Columbia Univ New York NY USA 10032 t & Cell Biol, New York, NY 10032 USA Columbia Univ, Ctr Neurobiol & Behav, New York, NY 10032 USA Columbia Univ New York NY USA 10032 obiol & Behav, New York, NY 10032 USA New York State Psychiat Inst, Dept Neurosci, New York, NY 10032 USA New York State Psychiat Inst New York NY USA 10032 New York, NY 10032 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 99, anno: 2000,
pagine: 445 - 456
SICI:
0306-4522(2000)99:3<445:MDNSRI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEUS-ACCUMBENS NEURONS; RECEPTOR-DEFICIENT MICE; STRIATUM IN-VIVO; RAT STRIATUM; ULTRASTRUCTURAL-LOCALIZATION; HIPPOCAMPAL-NEURONS; D2 RECEPTOR; BRAIN; TRANSMISSION; RELEASE;
Keywords:
ventral tegmental area; nucleus accumbens; glutamate; cotransmission; synaptic physiology; microculture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Rayport, S Columbia Univ, Dept Psychiat, New York, NY 10032 USA Columbia Univ New York NY USA 10032 at, New York, NY 10032 USA
Citazione:
M.P. Joyce e S. Rayport, "Mesoaccumbens dopamine neuron synapses reconstructed in vitro are glutamatergic", NEUROSCIENC, 99(3), 2000, pp. 445-456

Abstract

The mesoaccumbens projection, formed by ventral tegmental area dopamine neurons synapsing on nucleus accumbens gamma-aminobutyl ic acid neurons, has been implicated in the pathogenesis of schizophrenia and drug addiction. Despite intensive study, the nature of the signal conveyed by dopamine neurons has not been fully resolved. In addition to several slower, dopamine-mediated, modulatory actions, several lines of evidence suggest that dopamine neurons have fast excitatory actions. To rest this, we placed dopamine neurons together with accumbens neurons in microcultures. Surprisingly, most dopamine neurons made excitatory recurrent connections (autapses), which provided a basis for their identification; accumbens gamma-aminobutyric acid neurons were identified by their distinctive size. In 75% of mesoaccumbens cell pairs, stimulation of the dopamine neuron evoked a glutamate mediated, excitatory synaptic response in the accumbens neuron. Immunostaining revealeddopamine neuron varicosities that were predominantly dopaminergic, ones that were predominantly glutamatergic, and ones that were both dopaminergic and glutamatergic. Despite close appositions of both glutamatergic and dopaminergic varicosities to the dendrites of accumbens neurons, only glutamatergic synaptic responses were seen. In the majority of cell pairs, pharmacologic activation of D2-type dopamine receptors inhibited glutamatergic responses, presumably via immunocytochemically-visualized presynaptic D2 receptors. In some cell pairs, the evoked autaptic and synaptic responses were discordant, suggesting that D2 receptors may be differentially trafficked to different presynaptic varicosities. Thus, dopamine neurons appear to mediate both slow dopaminergic and fast glutamatergic actions via separate sets of synapses. Together with evidence for glutamate cotransmission in serotonergic raphe neurons and noradrenergic locus coeruleus neurons, these results add a new dimension to monoamine neuron signaling that may have important implications for neuropsychiatric disorders. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:10:18