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Titolo:
Transduction of murine cerebellar neurons with recombinant FIV and AAV5 vectors
Autore:
Alisky, JM; Hughes, SM; Sauter, SL; Jolly, D; Dubensky, TW; Staber, PD; Chiorini, JA; Davidson, BL;
Indirizzi:
Univ Iowa, Coll Med, Dept Internal Med, Program Gene Therapy, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 am Gene Therapy, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Dept Neurol, Program Gene Therapy, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 am Gene Therapy, Iowa City, IA 52242 USA Chiron Corp, Ctr Gene Therapy, San Diego, CA 92121 USA Chiron Corp San Diego CA USA 92121 Gene Therapy, San Diego, CA 92121 USA NIDCR, Gene Therapy & Therapeut Branch, Bethesda, MD 20892 USA NIDCR Bethesda MD USA 20892 py & Therapeut Branch, Bethesda, MD 20892 USA
Titolo Testata:
NEUROREPORT
fascicolo: 12, volume: 11, anno: 2000,
pagine: 2669 - 2673
SICI:
0959-4965(20000821)11:12<2669:TOMCNW>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; EFFICIENT TRANSDUCTION; NONDIVIDING CELLS; LENTIVIRAL VECTOR; PURKINJE-CELLS; VIRUS VECTORS; IN-VIVO; BRAIN; EXPRESSION; DELIVERY;
Keywords:
axonal transport; gene therapy; Purkinje cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Davidson, BL Univ Iowa, Coll Med, Dept Internal Med, Program Gene Therapy,Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 py, Iowa City, IA 52242 USA
Citazione:
J.M. Alisky et al., "Transduction of murine cerebellar neurons with recombinant FIV and AAV5 vectors", NEUROREPORT, 11(12), 2000, pp. 2669-2673

Abstract

Our data demonstrate that vectors derived from recombinant feline immunodeficiency virus (rFIV) and adeno-associated virus type 5 (rAAV5) transduce cerebellar cells following direct injection into the cerebellar lobules of mice. Both recombinant viruses mediated gene transfer predominantly to neurons, with up to 2500 and 1500 Purkinje cells transduced for rAAV5 or rFIV-based vectors, respectively. The vectors also transduced stellate, basket andGolgi neurons, with occasional transduction of granule cells and deep cerebellar nuclei. rAAV5 also spread outside the cerebellum to the inferior colliculus and ventricular epithelium, while rFIV demonstrated the ability to undergo retrograde transport to the physically close lateral vestibular nuclei. Thus, AAV5 and FIV-based vectors show promise for targeting neurons affected in the hereditary spinocerebellar ataxias. These vectors could be important tools for unraveling the pathophysiology of these disorders, or in testing factors which may promote neuronal survival. (C) 2000 Lippincott Williams & Wilkins.

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Documento generato il 14/07/20 alle ore 19:44:02