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Titolo:
OPPOSITE PARACRINE EFFECTS OF 5-HT AND DOPAMINE ON NA-PI COTRANSPORT IN OPOSSUM KIDNEY-CELLS()
Autore:
DETOLEDO FGS; BEERS KW; BERNDT TJ; THOMPSON MA; TYCE GM; KNOX FG; DOUSA TP;
Indirizzi:
MAYO CLIN & MAYO FDN,DEPT PHYSIOL,RENAL PATHOPHYSIOL LAB,921B GUGGENHEIM BLDG ROCHESTER MN 55905 MAYO CLIN & MAYO FDN,DEPT PHYSIOL,RENAL PATHOPHYSIOL LAB ROCHESTER MN55905 MAYO CLIN & MAYO FDN,DEPT BIOPHYS ROCHESTER MN 55905 MAYO CLIN & MAYO FDN,DEPT INTERNAL MED,NEPHROL RES UNIT ROCHESTER MN 55905
Titolo Testata:
Kidney international
fascicolo: 1, volume: 52, anno: 1997,
pagine: 152 - 156
SICI:
0085-2538(1997)52:1<152:OPEO5A>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
DECARBOXYLASE ACTIVITY; OK CELLS; 5-HYDROXYTRYPTAMINE; RECEPTORS; SLICES; RATS; LINE;
Keywords:
OPOSSUM KIDNEY CELLS; DOPAMINE; 5-HT; SODIUM-PHOSPHATE COTRANSPORT; TRANSPORT; CAMP SIGNALING PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
F.G.S. Detoledo et al., "OPPOSITE PARACRINE EFFECTS OF 5-HT AND DOPAMINE ON NA-PI COTRANSPORT IN OPOSSUM KIDNEY-CELLS()", Kidney international, 52(1), 1997, pp. 152-156

Abstract

Serotonin (5-HT) was recently reported to inhibit cAMP generation in oppossum (OK) cells. We thus investigated the effects of 5-HT upon theNa+-Pi cotransport in cultured OK cells and its interactions with dopamine. Incubation of OK cells with 1 nM-10 mu M 5-HT resulted in dose-dependent stimulation of Na+-Pi cotransport (ED50 congruent to 8 nM) and also counteracted inhibition of Na+-Pi cotransport elicited by dopamine. Pre-incubation with 5-HT decreased cAMP accumulation elicited byforskolin or dopamine and pre-treatment with pertussis toxin abolished both the inhibitory effect of 5-HT upon cAMP levels and stimulation of Na+-Pi cotransport. Incubation of OK cells with the 5-HT precursor 5-hydroxytryptophan resulted in time- and dose-dependent accumulation of 5-HT in the medium that also elicited an increase in Na+-Pi cotransport. Both the effects of 5-HT and dopamine on Na+-Pi cotransport wereprevented by carbidopa. The stimulatory effect of 5-HT was specific for the Na+-Pi cotransport system since no effects were observed on Na+-alanine cotransport. The results indicate that 5-HT stimulates Na+-Picotransport at least in part via inhibition of cAMP accumulation. We propose that 5-HT and dopamine have opposite actions as paracrine/autocrine regulators of Na+-Pi cotransport via opposite effects upon cAMP formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:37:24