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Titolo:
Mutations conferring amino acid residue substitutions in the carboxy-terminal domain of RNA polymerase alpha can suppress clpX and clpP with respect to developmentally regulated transcription in Bacillus subtilis
Autore:
Nakano, MM; Zhu, Y; Liu, JJ; Reyes, DY; Yoshikawa, H; Zuber, P;
Indirizzi:
Oregon Grad Inst Sci & Technol, Dept Biochem & Mol Biol, Beaverton, OR 97006 USA Oregon Grad Inst Sci & Technol Beaverton OR USA 97006 erton, OR 97006 USA Louisiana State Univ, Med Ctr, Dept Biochem & Mol Biol, Shreveport, LA 71130 USA Louisiana State Univ Shreveport LA USA 71130 ol, Shreveport, LA 71130 USA Tokyo Univ Agr, Dept Biosci, Tokyo 1568502, Japan Tokyo Univ Agr Tokyo Japan 1568502 gr, Dept Biosci, Tokyo 1568502, Japan
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 4, volume: 37, anno: 2000,
pagine: 869 - 884
SICI:
0950-382X(200008)37:4<869:MCAARS>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEIN; GENETIC COMPETENCE; ESCHERICHIA-COLI; MOLECULAR SWITCH; SIGMA-FACTOR; SRFA OPERON; NUCLEOTIDE-SEQUENCE; MU-TRANSPOSASE; DNA-BINDING; IN-VIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
88
Recensione:
Indirizzi per estratti:
Indirizzo: Zuber, P Oregon Grad Inst Sci & Technol, Dept Biochem & Mol Biol, 20000 NWWalker Rd, Beaverton, OR 97006 USA Oregon Grad Inst Sci & Technol 20000 NWWalker Rd Beaverton OR USA 97006
Citazione:
M.M. Nakano et al., "Mutations conferring amino acid residue substitutions in the carboxy-terminal domain of RNA polymerase alpha can suppress clpX and clpP with respect to developmentally regulated transcription in Bacillus subtilis", MOL MICROB, 37(4), 2000, pp. 869-884

Abstract

The Bacillus subtilis clpX and clpP genes are the sites of pleiotropic mutations that adversely affect growth on a variety of media and impair developmental processes such as sporulation and competence development. ClpX is necessary for the post-exponential induction of genes that require the sigma(H) form of RNA polymerase for transcription. Both ClpX and ClpP are required for the activation of sigma(A)-dependent transcription of the srf operonthat encodes surfactin synthetase and the regulatory peptide ComS, required for the development of genetic competence. Transcription of srf is activated by the two-component regulatory system ComPA in response to the peptidepheromone, ComX, which mediates cell density-dependent control. A clpX mutant, although able to produce ComX, is unable to respond to the pheromone. A mutant allele of comP, encoding a product whose activity is independent of ComX, is not able to suppress clpX with respect to srf expression, suggesting that ClpXP acts at the level of ComA-dependent activation of srf transcription initiation. Suppressor mutations of clpX (cxs-1 and cxs-2) were isolated in screens for pseudorevertants exhibiting high levels of srf expression and sigma(H)-dependent transcription respectively. One mutation, cxs-1, suppressed a clpP null mutation with respect to srf transcription, but did not overcome the block conferred by clpP on competence development and sporulation. Both cxs-1 and cxs-2 mutations map to the region of the rpoA gene encoding the RNA polymerase alpha C-terminal domain (alpha CTD). The reconstruction of the cxs-1 and cxs-2 alleles of rpoA confirmed that these mutations confer the suppressor phenotype. These findings provide further support for the hypothesis that ClpX and ClpP might be intimately associated with transcription initiation in B. subtilis.

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Documento generato il 25/11/20 alle ore 03:55:53