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Titolo:
Molecular analysis and chromosomal assignment of the canine CALC-I/alpha-CGRP gene
Autore:
Wende, S; Krempler, A; Breen, M; Brunnberg, L; Brenig, B;
Indirizzi:
Univ Gottingen, Inst Vet Med, D-37073 Gottingen, Germany Univ Gottingen Gottingen Germany D-37073 Med, D-37073 Gottingen, Germany Anim Hlth Trust, Newmarket CB8 7DW, Suffolk, England Anim Hlth Trust Newmarket Suffolk England CB8 7DW 8 7DW, Suffolk, England Univ Berlin, Clin Small Domest Anim, Berlin, Germany Univ Berlin Berlin Germany lin, Clin Small Domest Anim, Berlin, Germany
Titolo Testata:
MAMMALIAN GENOME
fascicolo: 9, volume: 11, anno: 2000,
pagine: 736 - 740
SICI:
0938-8990(200009)11:9<736:MAACAO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCITONIN ALPHA-CGRP; TESTICULAR DESCENT; TRINUCLEOTIDE REPEATS; MESSENGER-RNA; CRYPTORCHIDISM; EXPRESSION; PEPTIDE; MICE; INSENSITIVITY; FAMILIARIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Brenig, B Univ Gottingen, Inst Vet Med, Groner Landstr 2, D-37073 Gottingen, Germany Univ Gottingen Groner Landstr 2 Gottingen Germany D-37073 rmany
Citazione:
S. Wende et al., "Molecular analysis and chromosomal assignment of the canine CALC-I/alpha-CGRP gene", MAMM GENOME, 11(9), 2000, pp. 736-740

Abstract

We have isolated a recombinant phage harboring the canine CALC-I/alpha-CGRP gene. The gene spans a region of approx. 5.3 kb and consists of six exonswith sizes ranging from 95 bp (exon 2) and 494 bp (exon 4). By alternativesplicing, two transcripts with ORFs of 390 and 384 nt are generated. Theseencode either the 32-amino acid-long hormone calcitonin (CALC or the neurotransmitter calcitonin gene-related peptide (alpha-CGRP) with a length of 37 amino acids after proteolytic processing of precursor molecules. The canine calcitonin precursor consists of 130 amino acids with a molecular mass of 14.05 kDa and a statistical pi of 8.0, whereas the deduced alpha-CGRP precursor harbors 128 amino acids with a molecular mass of 13.87 kDa and a statistical pi of 8.6. Both polypeptides have a common N-terminal region of 76amino acids that is encoded by exons 2 and 3 and separated by different eight (CALC) or six (alpha-CGRP) amino acid spacers from the biologically active polypeptide. The CALC-I/alpha-CGRP gene is a member of the calcitonin gene family and was assigned to chromosome CFA 16q35.1. A comparative analysis of different dog breeds revealed a breed-specific allelic d(CAGGAG)-hexanucleotide expansion in exon 3. This expansion results in an elongation of the common N-terminal region by two amino acids (glutamine-glutamic acid) and alters the molecular mass to 14.31 kDa (pI 7.9) and 14.13 kDa (pI 8.5) of the calcitonin and alpha-CGRP precursor, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 00:40:42