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Titolo:
Abnormal formation of the glucan network from regenerating protoplasts in Schizosaccharomyces pombe cps8 actin point mutant
Autore:
Konomi, M; Ishiguro, J; Osumi, M;
Indirizzi:
Japan Womens Univ, Grad Sch Sci, Div Mat & Biol Sci, Bunkyo Ku, Tokyo 1128681, Japan Japan Womens Univ Tokyo Japan 1128681 i, Bunkyo Ku, Tokyo 1128681, Japan Japan Womens Univ, Dept Chem & Biol Sci, Fac Sci, Bunkyo Ku, Tokyo 1128681, Japan Japan Womens Univ Tokyo Japan 1128681 i, Bunkyo Ku, Tokyo 1128681, Japan Konan Univ, Fac Sci, Dept Biol, Kobe, Hyogo 6588501, Japan Konan Univ Kobe Hyogo Japan 6588501 Dept Biol, Kobe, Hyogo 6588501, Japan
Titolo Testata:
JOURNAL OF ELECTRON MICROSCOPY
fascicolo: 4, volume: 49, anno: 2000,
pagine: 569 - 578
SICI:
0022-0744(2000)49:4<569:AFOTGN>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-WALL FORMATION; SCANNING ELECTRON-MICROSCOPY; FISSION YEAST; SACCHAROMYCES-CEREVISIAE; VESICULAR TRANSPORT; SEPTUM FORMATION; HIGH-RESOLUTION; ULTRASTRUCTURE; CYTOSKELETON; MORPHOGENESIS;
Keywords:
ultra-high-resolution low-voltage scanning electron microscopy; cell wall formation; glucan fibrils; actin cytoskeleton; protoplast regeneration; Schizosaccharomyces pombe;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Osumi, M Japan Womens Univ, Grad Sch Sci, Div Mat & Biol Sci, Bunkyo Ku, 2-8-1 Mejirodai, Tokyo 1128681, Japan Japan Womens Univ 2-8-1 Mejirodai Tokyo Japan 1128681 681, Japan
Citazione:
M. Konomi et al., "Abnormal formation of the glucan network from regenerating protoplasts in Schizosaccharomyces pombe cps8 actin point mutant", J ELEC MICR, 49(4), 2000, pp. 569-578

Abstract

To study the close relationship between the actin cytoskeleton and cell wall formation, the process of cell wall formation in reverting protoplasts of the fission yeast, Schizosaccharomyces pombe, cps8 actin point mutant wasinvestigated by ultra-high-resolution low-voltage scanning electron microscopy (UHR-LVSEM) and transmission electron microscopy (TEM). The protoplastof the cps8 mutant began to form a glucan network in a unipolar manner andto secrete alpha-galactomannan. The site of cell wall formation grew in a cylindrical shape in the wild-type protoplast. The alpha-galactomannan did not fill in the intrafibrillar spaces completely, however, and the fibrils were exposed on the cell surface. UHR-LVSEM images indicated that the glucan fibrils were thin and rope-shaped, forming a looser network than the wild-type. TEM images indicated the finest fibrils were similar to 1.5 nm in diameter, the same diameter as the wild-type. These results suggest that the cps8 mutant was insufficient in developing cross-linkage with the glucan fibrils up to the wide ribbon shape as found in the wild-type [Osumi M et al. (1989) J. Electron Microsc. 38: 457-468; Osumi M (1998) Micron 29: 207-233]. These findings appear to indicate that the actin cytoskeleton controls formation of the glucan network and secretion of beta-1,6-glucan, and confirm the close relationship of the actin cytoskeleton and glucan formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 00:03:45