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Titolo:
Importance of membrane- or matrix-associated forms of M-CSF and RANKL/ODF in osteoclastogenesis supported by SaOS-4/3 cells expressing recombinant PTH/PTHrP receptors
Autore:
Itoh, K; Udagawa, N; Matsuzaki, K; Takami, M; Amano, H; Shinki, T; Ueno, Y; Takahashi, N; Suda, T;
Indirizzi:
Showa Univ, Sch Dent, Dept Biochem, Shinagawa Ku, Tokyo 1428555, Japan Showa Univ Tokyo Japan 1428555 ochem, Shinagawa Ku, Tokyo 1428555, Japan Showa Univ, Sch Dent, Dept Pharmacol, Tokyo 1428555, Japan Showa Univ Tokyo Japan 1428555 ent, Dept Pharmacol, Tokyo 1428555, Japan
Titolo Testata:
JOURNAL OF BONE AND MINERAL RESEARCH
fascicolo: 9, volume: 15, anno: 2000,
pagine: 1766 - 1775
SICI:
0884-0431(200009)15:9<1766:IOMOMF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; FACTOR-I; PROTEOGLYCAN FORM; OSTEOPROTEGERIN LIGAND; DIFFERENTIATION FACTOR; OSTEOBLASTIC CELLS; T-CELLS; MICE; IDENTIFICATION; DEFICIENCY;
Keywords:
osteoclastogenesis; macrophage colony-stimulating factor; receptor activator nuclear factor -kappa Beta ligand; parathyroid hormone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Suda, T Showa Univ, Sch Dent, Dept Biochem, Shinagawa Ku, 1-5-8 Hatanodai,Tokyo 1428555, Japan Showa Univ 1-5-8 Hatanodai Tokyo Japan 1428555 kyo 1428555, Japan
Citazione:
K. Itoh et al., "Importance of membrane- or matrix-associated forms of M-CSF and RANKL/ODF in osteoclastogenesis supported by SaOS-4/3 cells expressing recombinant PTH/PTHrP receptors", J BONE MIN, 15(9), 2000, pp. 1766-1775

Abstract

SaOS-4/3, a subclone of the human osteosarcoma cell line SaOS-2, established by transfecting the human parathyroid hormone/parathyroid hormone-related protein (PTH/PTHrP) receptor complementary DNA (cDNA), supported osteoclast formation in response to PTH in coculture with mouse bone marrow cells. Osteoclast formation supported by SaOS-4/3 cells was completely inhibited by adding either osteoprotegerin (OPG) or antibodies against human macrophage colony-stimulating factor (M-CSF). Expression of messenger RNAs (mRNAs) for receptor activator of NF-kappa B ligand/osteoclast differentiation factor (RANKL/ODF) and both membrane-associated and secreted forms of M-CSF by SaOS-4/3 cells was up-regulated in response to PTH, SaOS-4/3 cells constitutively expressed OPG mRNA, expression of which was down-regulated by PTH, Toelucidate the mechanism of PTH-induced osteoclastogenesis, SaOS-4/3 cells were spot-cultured for 2 h in the center of a culture well and then mouse bone marrow cells were uniformly plated over the well. When the spot coculture was treated for 6 days with both PTH and M-CSF, osteoclasts were inducedexclusively inside the colony of SaOS-4/3 cells, Osteoclasts were formed both inside and outside the colony of SaOS-4/3 cells in coculture treated with a soluble form of RANKL/ODF (sRANKL/sODF) in the presence of M-CSF. Whenthe spot coculture was treated with sRANKL/sODF, osteoclasts were formed only inside the colony of SaOS-4/3 cells. Adding M-CSF alone failed to support osteoclast formation in the spot coculture. PTH-induced osteoclast formation occurring inside the colony of SaOS-4/3 cells was not affected by the concentration of M-CSF in the culture medium. Mouse primary osteoblasts supported osteoclast formation in a similar fashion to SaOS-4/3 cells, These findings suggest that the up-regulation of RANKL/ODF expression is an essential step for PTH-induced osteoclastogenesis, and membrane- or matrix-associated forms of both M-CSF and RANKL/ODF are essentially involved in osteoclast formation supported by osteoblasts/stromal cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 06:55:55