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Titolo:
Persistent yeast single-stranded RNA viruses exist in vivo as genomic RNA center dot RNA polymerase complexes in 1 : 1 stoichiometry
Autore:
Solorzano, A; Rodriguez-Cousino, N; Esteban, R; Fujimura, T;
Indirizzi:
Univ Salamanca, CSIC, Inst Microbiol Bioquim, Dept Genet & Microbiol, Salamanca 37007, Spain Univ Salamanca Salamanca Spain 37007 & Microbiol, Salamanca 37007, Spain
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 34, volume: 275, anno: 2000,
pagine: 26428 - 26435
SICI:
0021-9258(20000825)275:34<26428:PYSRVE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
OPHIOSTOMA-NOVO-ULMI; ELM DISEASE FUNGUS; SACCHAROMYCES-CEREVISIAE; MESSENGER-RNA; MITOCHONDRIAL GENOME; HYPOVIRULENT STRAIN; MOLECULAR-CLONING; GENE-EXPRESSION; CELL-GROWTH; T-DSRNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Fujimura, T Univ Salamanca, CSIC, Inst Microbiol Bioquim, Dept Genet & Microbiol, AvdaCampo Ccharro S-N, Salamanca 37007, Spain Univ Salamanca Avda Campo Ccharro S-N Salamanca Spain 37007 n
Citazione:
A. Solorzano et al., "Persistent yeast single-stranded RNA viruses exist in vivo as genomic RNA center dot RNA polymerase complexes in 1 : 1 stoichiometry", J BIOL CHEM, 275(34), 2000, pp. 26428-26435

Abstract

Yeast narnavirus 20 S and 23 S RNAs encode RNA-dependent RNA polymerases p91 and p104, respectively, but do not encode coat proteins. Both RNAs form ribonucleoprotein complexes with their cognate polymerases. Here we show that these complexes are not localized in mitochondria, unlike the closely related mitoviruses, which reside in these organelles. Cytoplasmic localization of these polymerases was demonstrated by immunofluorescence and by fluorescence emitted from green fluorescent protein-fused polymerases. These fusion proteins were able to form ribonucleoprotein complexes as did the wild-type polymerases. Fluorescent observations and cell fractionation experiments suggested that the polymerases were stabilized by complex formation withtheir viral RNA genomes. Immunoprecipitation experiments with anti-green fluorescent protein antibodies demonstrated that a single polymerase molecule binds to a single viral RNA genome in the complex. Moreover, the majority(if not all) of 20 S and 23 S RNA molecules were found to form complexes with their cognate RNA polymerases. Since these viral RNAs were not encapsidated, ribonucleoprotein complex formation with their cognate RNA polymerases appears to be their strategy to survive in the host as persistent viruses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 05:02:05