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Titolo:
Prevotella enzymes involved in mucin oligosaccharide degradation and evidence for a small operon of genes expressed during growth on mucin
Autore:
Wright, DP; Rosendale, DI; Roberton, AM;
Indirizzi:
Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand Univ Auckland Auckland New Zealand 1 h Biol Sci, Auckland 1, New Zealand
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 1, volume: 190, anno: 2000,
pagine: 73 - 79
SICI:
0378-1097(20000901)190:1<73:PEIIMO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-COLON; BACTEROIDES-FRAGILIS; ULCERATIVE-COLITIS; TRICORN PROTEASE; PDZ DOMAIN; MUCUS; POLYSACCHARIDES; GLYCOPROTEINS; STRATEGIES; SULFATION;
Keywords:
mucin degradation; Prevotella; sulfatase; sulfomucin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Roberton, AM Univ Auckland, Sch Biol Sci, Private Bag 92019, Auckland 1, New Zealand Univ Auckland Private Bag 92019 Auckland New Zealand 1 aland
Citazione:
D.P. Wright et al., "Prevotella enzymes involved in mucin oligosaccharide degradation and evidence for a small operon of genes expressed during growth on mucin", FEMS MICROB, 190(1), 2000, pp. 73-79

Abstract

Mucin desulfation is believed to be a rate-limiting step in mucin degradation by colon bacteria. The activities of enzymes hydrolysing nine linkages found in mucin oligosaccharide chains were measured using model substrates,in extracts of two mucin-degrading bacteria, Prevotella strain RS2 and Bacteroides fragilis. Sulfatases desulfating N-acetylglucosamine-6-sulfate, galactose-6-sulfate and galactose-3-sulfate were found. The genomic DNA downstream from the gene encoding the mucin-desulfating sulfatase (N-acetylglucosamine-6-sulfatase) in Prevotella was sequenced, and two putative genes identified which are likely to be coexpressed with this sulfatase, though their activities are unknown. Northern and Western analyses showed that expression of this short operon of three genes is increased during growth on mucin. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 13:46:44