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Titolo:
Quantitative biochemical and ultrastructural comparison of mitochondrial permeability transition in isolated brain and liver mitochondria: Evidence for reduced sensitivity of brain mitochondria
Autore:
Berman, SB; Watkins, SC; Hastings, TG;
Indirizzi:
Univ Pittsburgh, Dept Neurosci, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 eurosci, Pittsburgh, PA 15213 USA Univ Pittsburgh, Dept Cell Biol & Physiol, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 Physiol, Pittsburgh, PA 15213 USA Univ Pittsburgh, Dept Neurol, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 Neurol, Pittsburgh, PA 15213 USA
Titolo Testata:
EXPERIMENTAL NEUROLOGY
fascicolo: 2, volume: 164, anno: 2000,
pagine: 415 - 425
SICI:
0014-4886(200008)164:2<415:QBAUCO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; CYCLOSPORINE-A; HEART-MITOCHONDRIA; CYTOCHROME-C; RAT-LIVER; NEURODEGENERATIVE DISEASES; PYRIDINE-NUCLEOTIDES; NERVOUS-SYSTEM; IN-VIVO; GLUTATHIONE;
Keywords:
mitochondria; permeability transition; neurodegeneration; apoptosis; excitotoxicity; oxidative stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Berman, SB Univ Pittsburgh, Dept Neurosci, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 ttsburgh, PA 15213 USA
Citazione:
S.B. Berman et al., "Quantitative biochemical and ultrastructural comparison of mitochondrial permeability transition in isolated brain and liver mitochondria: Evidence for reduced sensitivity of brain mitochondria", EXP NEUROL, 164(2), 2000, pp. 415-425

Abstract

Opening of the mitochondrial permeability transition pore has increasinglybeen implicated in excitotoxic, ischemic, and apoptotic cell death, as well as in several neurodegenerative disease processes. However, much of the work directly characterizing properties of the transition pore has been performed in isolated liver mitochondria. Because of suggestions of tissue-specific differences in pore properties, we directly compared isolated brain mitochondria with liver mitochondria and used three quantitative biochemical and ultrastructural measurements of permeability transition. We provide evidence that brain mitochondria do not readily undergo permeability transition upon exposure to conditions that rapidly induce the opening of the transition pore in liver mitochondria. Exposure of liver mitochondria to transition-inducing agents led to a large, cyclosporin A-inhibitable decrease in spectrophotometric absorbance, a loss of mitochondrial glutathione, and morphologic evidence of matrix swelling and disruption, as expected. However, wefound that similarly treated brain mitochondria showed very little absorbance change and no loss of glutathione, The absence of response in brain wasnot simply due to structural limitations, since large-amplitude swelling and release of glutathione occurred when membrane pores unrelated to the transition pore were formed. Additionally, electron microscopy revealed that the majority of brain mitochondria appeared morphologically unchanged following treatment to induce permeability transition. These findings show that isolated brain mitochondria are more resistant to induction of permeability transition than mitochondria from liver, which may have important implications for the study of the mechanisms involved in neuronal cell death. (C) 2000 Academic Press.

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Documento generato il 09/04/20 alle ore 07:35:11