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Titolo:
Differential modulation of AMPA receptors by cyclothiazide in two types ofstriatal neurons
Autore:
Vorobjev, VS; Sharonova, IN; Haas, HL; Sergeeva, OA;
Indirizzi:
Univ Dusseldorf, Dept Neurophysiol, D-40001 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-40001 , D-40001 Dusseldorf, Germany Russian Acad Med Sci, Brain Res Inst, Moscow 103064, Russia Russian Acad Med Sci Moscow Russia 103064 es Inst, Moscow 103064, Russia
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 8, volume: 12, anno: 2000,
pagine: 2871 - 2880
SICI:
0953-816X(200008)12:8<2871:DMOARB>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIUM SPINY NEURONS; GLUTAMATE RECEPTORS; CHOLINERGIC INTERNEURONS; CA2+ PERMEABILITY; RAT NEOSTRIATUM; SPLICE VARIANTS; BASAL GANGLIA; PATCH-CLAMP; DESENSITIZATION; EXPRESSION;
Keywords:
desensitization; glutamate receptors; patch-clamp; rat; RT-PCR; splice variant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Sergeeva, OA Univ Dusseldorf, Dept Neurophysiol, POB 101007, D-40001 Dusseldorf, Germany Univ Dusseldorf POB 101007 Dusseldorf Germany D-40001 ermany
Citazione:
V.S. Vorobjev et al., "Differential modulation of AMPA receptors by cyclothiazide in two types ofstriatal neurons", EUR J NEURO, 12(8), 2000, pp. 2871-2880

Abstract

The modulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazol-propionate (AMPA) receptor-mediated currents by cyclothiazide was investigated in acutelyisolated cells from rat striatum with whole-cell patch-clamp recording. Single-cell reverse transcriptase-polymerase chain reaction (RT-PCR) was usedto identify medium spiny and giant aspiny neurons and to determine their AMPA receptor subunit composition mostly in separate experiments. After pretreatment with cyclothiazide, kainate-induced AMPA responses were more strongly potentiated in medium spiny than in giant aspiny neurons; cyclothiazideinduced a ninefold leftward shift in the kainate concentration-response curve for medium spiny neurons (not giant aspiny neurons). The EC(50)s for the cyclothiazide potentiation did not differ substantially between medium spiny neurons and giant aspiny neurons. The recovery of kainate-activated currents from modulation by cyclothiazide was slower for medium spiny neurons than for giant aspiny neurons. Medium spiny neurons expressed GluR-A, GluR-B and GluR-C, but not GluR-D subunits in both flip and flop splice variants. All giant aspiny neurons expressed GluR-A and GluR-D, exclusively in the flop form, half of them also expressed GluR-B and GluR-C. This is in keeping with slow and fast desensitization kinetics in medium spiny neurons and giant aspiny neurons, respectively, and differences in cyclothiazide modulation. The rate of cyclothiazide dissociation from the AMPA receptor, activated by glutamate, was similar to 90 times slower in medium spiny neurons than in giant aspiny neurons. In giant aspiny neurons (not medium spiny neurons) this rate was strongly dependent on the presence of an agonist; 1 mm glutamate increased it 30-fold. Thus, two major cell groups in the striatum display distinct AMPA receptor compositions carrying specific properties of glutamate responses. Excitatory transmission will thus be differentially affected by cyclothiazide-type compounds.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 11:50:12