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Titolo:
Performance of Para-Pak (TM) Ultra ECOFIX (TM) compared with ParaPak (TM) Ultra formalin/mercuric chloride-based polyvinyl alcohol for concentration and permanent stained smears of stool parasites
Autore:
Fedorko, DP; Williams, EC; Nelson, NA; Mazyck, TD; Hanson, KL; Cartwright, CP;
Indirizzi:
NIH, Warren Grant Magnuson Clin Ctr, Microbiol Serv, Dept Clin Pathol, Bethesda, MD 20892 USA NIH Bethesda MD USA 20892 Serv, Dept Clin Pathol, Bethesda, MD 20892 USA Hennepin Cty Med Ctr, Clin Microbiol Lab, Minneapolis, MN 55415 USA Hennepin Cty Med Ctr Minneapolis MN USA 55415 , Minneapolis, MN 55415 USA
Titolo Testata:
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
fascicolo: 1, volume: 37, anno: 2000,
pagine: 37 - 39
SICI:
0732-8893(200005)37:1<37:POP(UE>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL PROTOZOAN MORPHOLOGY; SULFATE;
Keywords:
fecal preservative; stool specimens; intestinal parasites; protozoa; helminths;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Fedorko, DP NIH, Warren Grant Magnuson Clin Ctr, Microbiol Serv, Dept ClinPathol, Bldg 10,Room 2C385,10 Ctr Dr,MSC 1508, Bethesda, MD 20892 USA NIH Bldg 10,Room 2C385,10 Ctr Dr,MSC 1508 Bethesda MD USA 20892
Citazione:
D.P. Fedorko et al., "Performance of Para-Pak (TM) Ultra ECOFIX (TM) compared with ParaPak (TM) Ultra formalin/mercuric chloride-based polyvinyl alcohol for concentration and permanent stained smears of stool parasites", DIAG MICR I, 37(1), 2000, pp. 37-39

Abstract

ECOFIX(TM) is a mercury and formalin-free fecal preservative that can be used for concentration of stool specimens and preparation of permanently-stained slides. In this study, the standard two-vial ParaPak(TM) Ultra system was compared with ECOFIX(TM) Ultra for the detection of intestinal parasites. A total of 261 specimens in 92 sets (77 with 3 specimens, 15 with 2 specimens) were collected in ECOFIX(TM), formalin, and low viscosity polyvinyl alcohol (LV-PVA). Concentrations were performed from ECOFIX(TM) using Hemo-De and saline and from formalin using ethyl acetate and formalin. To prepare permanently-stained smears, ECOSTAIN(TM) (a modification of Wheatley's trichrome stain) was used on ECOFIX(TM) material and Wheatley's trichrome stain was used on specimens preserved in PVA. A total of 157 protozoa and helminths were detected; 132 (84.1%) were recovered in formalin/PVA and 129 (82.2%) in ECOFIX(TM). fn permanently-stained smears, 139 protozoa were observed, 116 (83.5%) in PVA-preserved material and 117 (84.2%) in ECOFIX(TM). Fecal concentration yielded 111 parasites (103 protozoa and 8 helminths), of which 98 (88.3%) were detected in formalin-fixed stool and 48 (43.2%) in ECOFIX(TM). Significantly fewer ECOFIX(TM)-preserved concentrates were positive for Blastocystis hominis (35 versus 15, p-value <0.001) and Endolimax nana (19 versus 2, p-value <0.001). In conclusion, use of the ECOFIX(TM) Ultra collection device in combination with ECOSTAIN(TM) resulted in largely comparable recovery of enteric parasites to the conventional two-vial ParaPak(TM) Ultra system when both sedimentation-concentration and permanently stained smears were performed, and 2-3 specimens per patient were evaluated. (C) 2000 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 06:39:16