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Titolo:
IGFs, insulin, Shh, bFGF, and TGF-beta 1 interact synergistically to promote somite myogenesis in vitro
Autore:
Pirskanen, A; Kiefer, JC; Hauschka, SD;
Indirizzi:
Univ Washington, Dept Biochem, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Biochem, Seattle, WA 98195 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 224, anno: 2000,
pagine: 189 - 203
SICI:
0012-1606(20000815)224:2<189:IISBAT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; SKELETAL-MUSCLE DIFFERENTIATION; ACTIVATED PROTEIN-KINASE; BHLH GENE-EXPRESSION; DORSAL NEURAL-TUBE; SONIC HEDGEHOG; PHOSPHATIDYLINOSITOL 3-KINASE; MYOBLAST DIFFERENTIATION; PARAXIAL MESODERM; FACTOR SYSTEM;
Keywords:
somite; myogenesis; muscle; neural tube; insulin-like growth factors (IGFs); IGF receptors; Sonic hedgehog (Shh); fibroblast growth factor; TGF-beta 1; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Hauschka, SD Univ Washington, Dept Biochem, Box 357350, Seattle, WA 98195 USA Univ Washington Box 357350 Seattle WA USA 98195 WA 98195 USA
Citazione:
A. Pirskanen et al., "IGFs, insulin, Shh, bFGF, and TGF-beta 1 interact synergistically to promote somite myogenesis in vitro", DEVELOP BIO, 224(2), 2000, pp. 189-203

Abstract

Studies from our group and others have shown that in vitro somite myogenesis is regulated by neural tube and notochord factors including Wnt, Sonic hedgehog (Shh), and basic fibroblast growth factor (bFGF) together with transforming growth factor-beta 1 (TGF-beta 1). In this study we report that insulin and insulin-like growth factors I and II (IGF-I and -II) also promotemyogenesis in explant cultures containing single somites or somite-sized pieces of segmental plate mesoderm from 2-day (stage 10-14) chicken embryos and that the combination of insulin/IGFs with bFGF plus TGF-beta 1 promoteseven higher levels of myogenesis. We also found that Shh promotes myogenesis in this in vitro system and that Shh interacts synergistically with insulin/IGFs to promote high levels of myogenesis. RT-PCR analysis detected insulin, IGF-II, insulin receptor, and IGF receptor mRNAs in both the neural tube and the somites, whereas IGF-I transcripts were detected in entire embryos but not in the neural tube or somites. Treatment of somite-neural tube cocultures with anti-insulin, anti-IGF-II, anti-insulin receptor, or anti-IGF receptor blocking antibodies caused a significant decrease in myogenesis. These results are consistent with the hypothesis that systemic IGF-I as well as insulin and IGF-II secreted by the neural tube act as additional early myogenic signals during embryogenesis. Further studies indicate that insulin, IGFs, bFGF, and Shh also stimulate somite cell proliferation and influence apoptosis. (C) 2000 Academic Press.

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Documento generato il 25/09/20 alle ore 00:18:33