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Titolo:
Effect of nitric oxide and nitric oxide donors on red blood cell oxygen transport
Autore:
Hrinczenko, BW; Alayash, AI; Wink, DA; Gladwin, MT; Rodgers, GP; Schechter, AN;
Indirizzi:
NIDDK, NIH, Biol Chem Lab, Bethesda, MD 20892 USA NIDDK Bethesda MD USA 20892 K, NIH, Biol Chem Lab, Bethesda, MD 20892 USA US FDA, Ctr Biol Evaluat & Res, Div Hematol, Lab Plasma Derivat, Bethesda,MD USA US FDA Bethesda MD USA Div Hematol, Lab Plasma Derivat, Bethesda,MD USA NCI, NIH, Radiat Biol Branch, Div Clin Sci, Bethesda, MD USA NCI BethesdaMD USA , Radiat Biol Branch, Div Clin Sci, Bethesda, MD USA NIH, Ctr Clin, Dept Crit Care Med, Bethesda, MD USA NIH Bethesda MD USANIH, Ctr Clin, Dept Crit Care Med, Bethesda, MD USA NIDDK, NIH, Mol & Clin Hematol Branch, Bethesda, MD 20892 USA NIDDK Bethesda MD USA 20892 & Clin Hematol Branch, Bethesda, MD 20892 USA
Titolo Testata:
BRITISH JOURNAL OF HAEMATOLOGY
fascicolo: 2, volume: 110, anno: 2000,
pagine: 412 - 419
SICI:
0007-1048(200008)110:2<412:EONOAN>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
S-NITROSOHEMOGLOBIN; COVALENT BINDING; HEMOGLOBIN-S; DISEASE; NO; HYDROXYUREA; AFFINITY; ERYTHROCYTES; GLUTATHIONE; OXIDATION;
Keywords:
nitric oxide; haemoglobin; methaemoglobin; oxygen affinity; sickle cell anaemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Schechter, AN NIDDK, NIH, Biol Chem Lab, Bldg 10,Rm 9N-307, Bethesda, MD 20892 USA NIDDK Bldg 10,Rm 9N-307 Bethesda MD USA 20892 , MD 20892 USA
Citazione:
B.W. Hrinczenko et al., "Effect of nitric oxide and nitric oxide donors on red blood cell oxygen transport", BR J HAEM, 110(2), 2000, pp. 412-419

Abstract

A mechanism has been proposed in which nitric oxide (NO) may bind to cysteine beta 93 and be transported B haemoglobin from the lungs to the tissues and modify vascular tone. In addition, it has been reported that treatment of sickle cell anaemia blood with 80 p.p.m. NO gas in air shifts the oxygenaffinity as measured by P-50 to the left. We exposed normal and sickle cell anaemia blood to 80 p.p.m. NO in air for 1 h in vitro and found no changein P-50 of either normal or sickle cell blood, in addition, we exposed normal and sickle cell blood in buffer to aqueous NO (NO gas dissolved in buffer) at varying concentrations and found that the induced left shift in P-50correlates strongly and linearly with methaemoglobin formation. We also treated normal and sickle cell blood with other nitric oxide donors, such as sodium 2-(N,N-diethylamino)-diazenolate-2-oxide (DEANO), S-nitrosocysteine (CysNO) and sodium trioxodinitrate (OXINO, or Angeli's salt). In all cases,we found a dose-dependent increase in methaemoglobin that was strongly correlated with the dose-dependent P-50 reduction. Our data do not support thereport that low NO concentrations call selectively increase the oxygen affinity of sickle fell blood without affecting methaemoglobin levels significantly. NO, however map have benefit in sickle cell disease by other mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:04:52