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Titolo:
Organization of transport from endoplasmic reticulum to Golgi in higher plants
Autore:
Andreeva, AV; Zheng, H; Saint-Jore, CM; Kutuzov, MA; Evans, DE; Hawes, CR;
Indirizzi:
Oxford Brookes Univ, Res Sch Biol & Mol Sci, Oxford OX3 0BP, England Oxford Brookes Univ Oxford England OX3 0BP Sci, Oxford OX3 0BP, England
Titolo Testata:
BIOCHEMICAL SOCIETY TRANSACTIONS
, volume: 28, anno: 2000,
parte:, 4
pagine: 505 - 512
SICI:
0300-5127(200008)28:<505:OOTFER>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR TRANSPORT; VESICLE TRAFFICKING; BREFELDIN-A; PROTEIN-TRANSPORT; GTP HYDROLYSIS; COAT PROTEINS; RAS P21; ER; YEAST; APPARATUS;
Keywords:
green fluorescent protein; mutagenesis; Sar1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Andreeva, AV Oxford Brookes Univ, Res Sch Biol & Mol Sci, Gipsy Lane Campus, Oxford OX30BP, England Oxford Brookes Univ Gipsy Lane Campus Oxford England OX3 0BP
Citazione:
A.V. Andreeva et al., "Organization of transport from endoplasmic reticulum to Golgi in higher plants", BIOCH SOC T, 28, 2000, pp. 505-512

Abstract

In plant cells, the organization of the Golgi apparatus and its interrelationships with the endoplasmic reticulum differ from those in mammalian and yeast cells. Endoplasmic reticulum and Golgi apparatus can now be visualized in plant cells in vivo with green fluorescent protein (GFP) specifically directed to these compartments. This makes it possible to study the dynamics of the membrane transport between these two organelles in the living cells. The GFP approach, in conjunction with a considerable volume of data about proteins participating in the transport between endoplasmic reticulum andGolgi in yeast and mammalian cells and the identification of their putative plant homologues, should allow the establishment of an experimental modelin which to test the involvement of the candidate proteins in plants. As afirst step towards the development of such a system, we are using Sari, a small G-protein necessary for vesicle budding from the endoplasmic reticulum. This work has demonstrated that the introduction of Sar1 mutants blocks the transport from endoplasmic reticulum to Golgi in vivo in tobacco leaf epidermal cells and has therefore confirmed the feasibility of this approachto test the function of other proteins that are presumably involved in this step of endomembrane trafficking in plant cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 10:04:55