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Titolo: Synaptic concentration of dopamine in rat striatal slices in relationship to [H-3]raclopride binding to the dopamine D-2 receptor
Autore: Park, MH; Park, EH;
- Indirizzi:
- Sookmyung Womens Univ, Coll Pharm, Yongsan Ku, Seoul 140742, South Korea Sookmyung Womens Univ Seoul South Korea 140742 Seoul 140742, South Korea
- Titolo Testata:
- ARCHIVES OF PHARMACAL RESEARCH
fascicolo: 4,
volume: 23,
anno: 2000,
pagine: 360 - 366
- SICI:
- 0253-6269(200008)23:4<360:SCODIR>2.0.ZU;2-8
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- POSITRON EMISSION TOMOGRAPHY; C-11 RACLOPRIDE BINDING; ENDOGENOUS DOPAMINE; H-3 RACLOPRIDE; KINETIC-PROPERTIES; HIGH-AFFINITY; D-AMPHETAMINE; HUMAN-BRAIN; PET; EQUILIBRIUM;
- Keywords:
- dopamine; [H-3]raclopride; striatal slice; electrical stimulation; amphetamine; alpha-methyl-p-tyrosine;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Life Sciences
- Citazioni:
- 20
- Recensione:
- Indirizzi per estratti:
- Indirizzo: Park, EH Sookmyung Womens Univ, Coll Pharm, Yongsan Ku, Chungpa Dong 2 Ka,Seoul 140742, South Korea Sookmyung Womens Univ Chungpa Dong 2 Ka Seoul South Korea 140742
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- Citazione:
- M.H. Park e E.H. Park, "Synaptic concentration of dopamine in rat striatal slices in relationship to [H-3]raclopride binding to the dopamine D-2 receptor", ARCH PH RES, 23(4), 2000, pp. 360-366
Abstract
The in vivo binding of dopamine (DA) radioligands to D-2 receptors can be affected by competition with endogenous dopamine. In the present study, we used a brain slice preparation that provides more controlled conditions than in vivo preparations in order to examine the relationship between synaptic DA and the binding of [H-3]raclopride to D-2 receptors. We also estimatedthe synaptic DA concentration in rat striatal slices by determining the changes in [H-3]raclopride binding. To correlate the changes in [H-3]raclopride binding with the concentration of synaptic DA, the kinetic parameters were determined. [H-3]Raclopride reached equilibrium binding conditions within two hours. The K-i value for DA in inhibiting [H-3]raclopride binding wasabout 2.2 nM. The increase in synaptic DA evoked by electrical stimulationdecreased the striatal binding of [H-3]raclopride in a frequency-dependentmanner. Increases in the DA concentration evoked by amphetamine (AMPH) or cocaine decreased [H-3]raclopride binding by 74% or 20%, respectively, corresponding to increases in the synaptic DA concentrations of 1.6 nM or 0.6 nM, respectively. Pargyline also decreased [H-3]raclopride binding by 36% corresponding at a concentration of 1.2 nM. In contrast, the depletion-of synaptic DA by alpha-methyl-p-tyrosine (alpha-MpT) increased the specific binding of [H-3]raclopride by 43% when the DA concentration was decreased to 0.7 nM. The changes in the DA concentration at the synapse were responsible for the changes in the striatal binding of [H-3]raclopride. The values calculated in this study may therefore approximate the changes in the synaptic DA concentration in rat striatal slices following manipulation.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:33:05