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Titolo:
Synaptic concentration of dopamine in rat striatal slices in relationship to [H-3]raclopride binding to the dopamine D-2 receptor
Autore:
Park, MH; Park, EH;
Indirizzi:
Sookmyung Womens Univ, Coll Pharm, Yongsan Ku, Seoul 140742, South Korea Sookmyung Womens Univ Seoul South Korea 140742 Seoul 140742, South Korea
Titolo Testata:
ARCHIVES OF PHARMACAL RESEARCH
fascicolo: 4, volume: 23, anno: 2000,
pagine: 360 - 366
SICI:
0253-6269(200008)23:4<360:SCODIR>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON EMISSION TOMOGRAPHY; C-11 RACLOPRIDE BINDING; ENDOGENOUS DOPAMINE; H-3 RACLOPRIDE; KINETIC-PROPERTIES; HIGH-AFFINITY; D-AMPHETAMINE; HUMAN-BRAIN; PET; EQUILIBRIUM;
Keywords:
dopamine; [H-3]raclopride; striatal slice; electrical stimulation; amphetamine; alpha-methyl-p-tyrosine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Park, EH Sookmyung Womens Univ, Coll Pharm, Yongsan Ku, Chungpa Dong 2 Ka,Seoul 140742, South Korea Sookmyung Womens Univ Chungpa Dong 2 Ka Seoul South Korea 140742
Citazione:
M.H. Park e E.H. Park, "Synaptic concentration of dopamine in rat striatal slices in relationship to [H-3]raclopride binding to the dopamine D-2 receptor", ARCH PH RES, 23(4), 2000, pp. 360-366

Abstract

The in vivo binding of dopamine (DA) radioligands to D-2 receptors can be affected by competition with endogenous dopamine. In the present study, we used a brain slice preparation that provides more controlled conditions than in vivo preparations in order to examine the relationship between synaptic DA and the binding of [H-3]raclopride to D-2 receptors. We also estimatedthe synaptic DA concentration in rat striatal slices by determining the changes in [H-3]raclopride binding. To correlate the changes in [H-3]raclopride binding with the concentration of synaptic DA, the kinetic parameters were determined. [H-3]Raclopride reached equilibrium binding conditions within two hours. The K-i value for DA in inhibiting [H-3]raclopride binding wasabout 2.2 nM. The increase in synaptic DA evoked by electrical stimulationdecreased the striatal binding of [H-3]raclopride in a frequency-dependentmanner. Increases in the DA concentration evoked by amphetamine (AMPH) or cocaine decreased [H-3]raclopride binding by 74% or 20%, respectively, corresponding to increases in the synaptic DA concentrations of 1.6 nM or 0.6 nM, respectively. Pargyline also decreased [H-3]raclopride binding by 36% corresponding at a concentration of 1.2 nM. In contrast, the depletion-of synaptic DA by alpha-methyl-p-tyrosine (alpha-MpT) increased the specific binding of [H-3]raclopride by 43% when the DA concentration was decreased to 0.7 nM. The changes in the DA concentration at the synapse were responsible for the changes in the striatal binding of [H-3]raclopride. The values calculated in this study may therefore approximate the changes in the synaptic DA concentration in rat striatal slices following manipulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/21 alle ore 16:33:05