Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Proteolytic degradation of tyrosine nitrated proteins
Autore:
Souza, JM; Choi, I; Chen, QP; Weisse, M; Daikhin, E; Yudkoff, M; Obin, M; Ara, J; Horwitz, J; Ischiropoulos, H;
Indirizzi:
Childrens Hosp Philadelphia, Div Neonatol, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA Childrens Hosp Philadelphia, Div Child Dev & Rehabil Med, Philadelphia, PA19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 elphia, PA19104 USA Childrens Hosp Philadelphia, Div Biochem & Biophys, Stokes Res Inst, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA Childrens Hosp Philadelphia, Dept Pediat, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia Philadelphia PA USA 19104 lphia, PA 19104 USA Univ Penn, Abramson Res Ctr 416D, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Ctr 416D, Philadelphia, PA 19104 USA Tufts Univ, Jean Mayer USDA, Lab Nutr & Vis Res, Human Nutr Res Ctr Aging,Boston, MA 02111 USA Tufts Univ Boston MA USA 02111 an Nutr Res Ctr Aging,Boston, MA 02111 USA Med Coll Penn & Hahnemann Univ, Sch Med, Dept Pharmacol, Philadelphia, PA 19102 USA Med Coll Penn & Hahnemann Univ Philadelphia PA USA 19102 ia, PA 19102 USA Univ Republ, Fac Med, Dept Bioquim, Montevideo 11800, Uruguay Univ RepublMontevideo Uruguay 11800 Bioquim, Montevideo 11800, Uruguay
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 2, volume: 380, anno: 2000,
pagine: 360 - 366
SICI:
0003-9861(20000815)380:2<360:PDOTNP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPEROXIDE-DISMUTASE; OXIDATIVE STRESS; NITRIC-OXIDE; 26S PROTEASOME; PEROXYNITRITE; PHOSPHORYLATION; 20S; INACTIVATION; ACTIVATION; DEATH;
Keywords:
3-nitrotyrosine; proteasome; chymotrypsin; superoxide dismutase; tyrosine hydroxylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Ischiropoulos, H Childrens Hosp Philadelphia, Div Neonatol, 34th St & Civic Ctr Blvd, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia 34th St & Civic Ctr Blvd Philadelphia PA USA 19104
Citazione:
J.M. Souza et al., "Proteolytic degradation of tyrosine nitrated proteins", ARCH BIOCH, 380(2), 2000, pp. 360-366

Abstract

Tyrosine nitration is a covalent posttranslational protein modification that has been detected under several pathological conditions. This study reports that nitrated proteins are degraded by chymotrypsin and that protein nitration enhances susceptibility to degradation by the proteasome. Chymotrypsin cleaved the peptide bond between nitrated-tyrosine 108 and serine 109 in bovine Cu,Zn superoxide dismutase. However, the rate of chymotryptic cleavage of nitrated peptides was considerably slower than control. In contrast, nitrated bovine Cu,Zn superoxide dismutase was degraded at a rate 1.8-fold faster than that of control by a gradient-purified 20S/26S proteasome fraction from bovine retina. Exposure of PC12 cells to a nitrating agent resulted in the nitration of tyrosine hydroxylase and a 58 +/- 12.5% decline in the steady-state levels of the protein 4 h after nitration. The steady-state levels of tyrosine hydroxylase were restored by selective inhibition of the proteasome activity with lactacystin. These data indicate that nitrationof tyrosine residue(s) in proteins is sufficient to induce an accelerated degradation of the modified proteins by the proteasome and that the proteasome may be critical for the removal of nitrated proteins in vivo. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 13:24:20